1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.

J T Troelsen; A Mehlum; N Spodsberg; H Prydz; O Norén; H Sjöström; Jørgen Olsen; Gert Helge Hansen

doi:10.1016/0014-5793(94)80519-9

1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.

J T Troelsen, A Mehlum, N Spodsberg, H Prydz, O Norén, H Sjöström, Jørgen Olsen, Gert Helge Hansen

Department of Cellular and Molecular Medicine

45 Citations (Scopus)

Abstract

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

Original language	English
Journal	FEBS Letters
Volume	342
Issue number	3
Pages (from-to)	291-6
Number of pages	5
ISSN	0014-5793
DOIs	https://doi.org/10.1016/0014-5793(94)80519-9
Publication status	Published - 1994

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10.1016/0014-5793(94)80519-9

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title = "1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.",

abstract = "Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.",

author = "Troelsen, {J T} and A Mehlum and N Spodsberg and H Prydz and O Nor{\'e}n and H Sj{\"o}str{\"o}m and J{\o}rgen Olsen and Hansen, {Gert Helge}",

note = "Keywords: Age Factors; Animals; Gene Expression Regulation; Glycosylceramidase; Intestine, Small; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Transgenic; Promoter Regions (Genetics); RNA, Messenger; Tissue Distribution; Transcription, Genetic",

year = "1994",

doi = "10.1016/0014-5793(94)80519-9",

language = "English",

volume = "342",

pages = "291--6",

journal = "F E B S Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "3",

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TY - JOUR

T1 - 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.

AU - Troelsen, J T

AU - Mehlum, A

AU - Spodsberg, N

AU - Prydz, H

AU - Norén, O

AU - Sjöström, H

AU - Olsen, Jørgen

AU - Hansen, Gert Helge

N1 - Keywords: Age Factors; Animals; Gene Expression Regulation; Glycosylceramidase; Intestine, Small; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Mice, Transgenic; Promoter Regions (Genetics); RNA, Messenger; Tissue Distribution; Transcription, Genetic

PY - 1994

Y1 - 1994

N2 - Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

AB - Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin hydrolase (LPH) reducing the intestinal capacity to hydrolyze lactose. We here demonstrate that the stretch -17 to -994 in the pig LPH-promoter carries cis-elements which direct a small intestinal-specific expression and a post-weaning decline of a linked rabbit beta-globin gene. These data demonstrate that the post-weaning decline of LPH is mainly due to a transcriptional down-regulation.

U2 - 10.1016/0014-5793(94)80519-9

DO - 10.1016/0014-5793(94)80519-9

M3 - Journal article

C2 - 8150087

SN - 0014-5793

VL - 342

SP - 291

EP - 296

JO - F E B S Letters

JF - F E B S Letters

IS - 3

ER -

1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice.

Abstract

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