Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

Nicolai B Larsen; Ian D Hickson; Hocine W Mankouri

doi:10.4161/15384101.2014.958912

Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

Nicolai B Larsen, Ian D Hickson, Hocine W Mankouri

7 Citationer (Scopus)

Abstract

The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

Originalsprog	Engelsk
Tidsskrift	Cell Cycle
Vol/bind	13
Udgave nummer	19
Sider (fra-til)	2994-8
Antal sider	5
ISSN	1538-4101
DOI	https://doi.org/10.4161/15384101.2014.958912
Status	Udgivet - 1 okt. 2014

Adgang til dokumentet

10.4161/15384101.2014.958912

Citationsformater

@article{f8bfbc6b4def468f8a041b9bf0637148,

title = "Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes",

abstract = "The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.",

author = "Larsen, {Nicolai B} and Hickson, {Ian D} and Mankouri, {Hocine W}",

year = "2014",

month = oct,

day = "1",

doi = "10.4161/15384101.2014.958912",

language = "English",

volume = "13",

pages = "2994--8",

journal = "Cell Cycle",

issn = "1538-4101",

publisher = "Taylor & Francis",

number = "19",

}

TY - JOUR

T1 - Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

AU - Larsen, Nicolai B

AU - Hickson, Ian D

AU - Mankouri, Hocine W

PY - 2014/10/1

Y1 - 2014/10/1

N2 - The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

AB - The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

U2 - 10.4161/15384101.2014.958912

DO - 10.4161/15384101.2014.958912

M3 - Journal article

C2 - 25486560

SN - 1538-4101

VL - 13

SP - 2994

EP - 2998

JO - Cell Cycle

JF - Cell Cycle

IS - 19

ER -

Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater