Abstract
The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.
| Original language | English |
|---|---|
| Journal | Cell Cycle |
| Volume | 13 |
| Issue number | 19 |
| Pages (from-to) | 2994-8 |
| Number of pages | 5 |
| ISSN | 1538-4101 |
| DOIs | |
| Publication status | Published - 1 Oct 2014 |