The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

TY - JOUR

T1 - The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress

AU - Clausing, Emanuel

AU - Mayer, Andreas

AU - Chanarat, Sittinan

AU - Müller, Barbara

AU - Germann, Susanne M

AU - Cramer, Patrick

AU - Lisby, Michael

AU - Strässer, Katja

PY - 2010/12/31

Y1 - 2010/12/31

N2 - Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNAbinding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-ΔC mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.

AB - Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNAbinding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-ΔC mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.

KW - Alleles

KW - Cyclin-Dependent Kinases

KW - Cyclins

KW - DNA Damage

KW - DNA Replication

KW - Genome

KW - Genome-Wide Association Study

KW - Microscopy, Fluorescence

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Protein Interaction Mapping

KW - Protein Structure, Tertiary

KW - Recombination, Genetic

KW - Replication Protein A

KW - Saccharomyces cerevisiae Proteins

KW - Temperature

U2 - 10.1074/jbc.M110.193292

DO - 10.1074/jbc.M110.193292

M3 - Journal article

C2 - 21075850

SN - 0021-9258

VL - 285

SP - 41665

EP - 41674

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 53

ER -