The effect of bacteriocin-producing Lactobacillus plantarum strains on the intracellular pH of sessile and planktonic Listeria monocytongenes single cells

TY - JOUR

T1 - The effect of bacteriocin-producing Lactobacillus plantarum strains on the intracellular pH of sessile and planktonic Listeria monocytongenes single cells

AU - Nielsen, Dennis Sandris

AU - Cho, Gyu-Sung

AU - Hanak, Alexander

AU - Huch, Melanie

AU - Franz, Charles M.A.P.

AU - Arneborg, Nils

PY - 2010/7

Y1 - 2010/7

N2 - A wide range of lactic acid bacteria (LAB) produce bacteriocins mainly active against other closely related LAB, but some bacteriocins are also active against the food-borne pathogen Listeria monocytogenes. With the aim of increasing food safety it has thus been considered to utilise bacteriocins and/or bacteriocin-producing LAB as “natural” food preservatives in foods such as cheese, meat and ready-to-eat products. Some strains of Lactobacillus plantarum produce bacteriocins termed plantaricins. Using a single-cell based approach, the effect on the intracellular pH as a measure of the physiological state of sessile and planktonic L. monocytogenes (strains EGDe and N53-1) during co-culturing with plantaricin-producing L. plantarum (strains BFE 5092 and PCS 20) was investigated using fluorescence ratio imaging microscopy (FRIM). Mono-cultures of L. monocytogenes were used as control. Expression levels of plantaricin-encoding genes by sessile and planktonic L. plantarum were determined using qRT-PCR. L. plantarum BFE 5092 possesses the genes for plantaricin EF, JK and N, while L. plantarum PCS 20 contains the genes for plantaricin EF, although determination of the nucleotide sequence of the PCS 20 plantaricin E gene showed that this peptide is probably non-functional. When cultured as mono-culture, both L. monocytogenes strains maintained pHi at a constant level around 7.2–7.6 throughout the experiment, independently of the matrix. On a solid surface, L. plantarum BFE 5092 strongly affected pHi of L. monocytogenes N53-1 with only 20% of the cells being able to maintain pHi in the physiological optimal range with pH > 7 and 52% of the cells with pHi ~ pHex, showing that the cells had no proton gradient towards the environment. The effect on L. monocytogenes EGDe was less pronounced, but still notable. L. plantarum PCS 20 left both strains of L. monocytogenes virtually unaffected when co-cultured on a solid surface. In liquid, both L. plantarum strains strongly affected the physiological state of L. monocytogenes EGDe as judged by pHi, whereas L. monocytogenes N53-1 was left virtually unaffected after 5 h of co-culturing and after 8 h 50% of the cells still maintained pHi = 7. Higher concentrations of lactic acid were produced in liquid compared to a solid surface, and the different response of EGDe and N53-1 to the activities of the two L. plantarum strains probably reflect higher susceptibility of L. monocytogenes EGDe to organic acids compared to L. monocytogenes N53-1. Taken together, our results may be explained by the difference in the range of plantaricins produced by the two L. plantarum strains and matrix- and strain-related differences in the susceptibility of L. monocytogenes to plantaricins and organic acids. In conclusion, the present study represents the first demonstration of the ability of a bacteriocin-producing LAB to dissipate the proton gradient of sessile and planktonic L. monocytogenes.

AB - A wide range of lactic acid bacteria (LAB) produce bacteriocins mainly active against other closely related LAB, but some bacteriocins are also active against the food-borne pathogen Listeria monocytogenes. With the aim of increasing food safety it has thus been considered to utilise bacteriocins and/or bacteriocin-producing LAB as “natural” food preservatives in foods such as cheese, meat and ready-to-eat products. Some strains of Lactobacillus plantarum produce bacteriocins termed plantaricins. Using a single-cell based approach, the effect on the intracellular pH as a measure of the physiological state of sessile and planktonic L. monocytogenes (strains EGDe and N53-1) during co-culturing with plantaricin-producing L. plantarum (strains BFE 5092 and PCS 20) was investigated using fluorescence ratio imaging microscopy (FRIM). Mono-cultures of L. monocytogenes were used as control. Expression levels of plantaricin-encoding genes by sessile and planktonic L. plantarum were determined using qRT-PCR. L. plantarum BFE 5092 possesses the genes for plantaricin EF, JK and N, while L. plantarum PCS 20 contains the genes for plantaricin EF, although determination of the nucleotide sequence of the PCS 20 plantaricin E gene showed that this peptide is probably non-functional. When cultured as mono-culture, both L. monocytogenes strains maintained pHi at a constant level around 7.2–7.6 throughout the experiment, independently of the matrix. On a solid surface, L. plantarum BFE 5092 strongly affected pHi of L. monocytogenes N53-1 with only 20% of the cells being able to maintain pHi in the physiological optimal range with pH > 7 and 52% of the cells with pHi ~ pHex, showing that the cells had no proton gradient towards the environment. The effect on L. monocytogenes EGDe was less pronounced, but still notable. L. plantarum PCS 20 left both strains of L. monocytogenes virtually unaffected when co-cultured on a solid surface. In liquid, both L. plantarum strains strongly affected the physiological state of L. monocytogenes EGDe as judged by pHi, whereas L. monocytogenes N53-1 was left virtually unaffected after 5 h of co-culturing and after 8 h 50% of the cells still maintained pHi = 7. Higher concentrations of lactic acid were produced in liquid compared to a solid surface, and the different response of EGDe and N53-1 to the activities of the two L. plantarum strains probably reflect higher susceptibility of L. monocytogenes EGDe to organic acids compared to L. monocytogenes N53-1. Taken together, our results may be explained by the difference in the range of plantaricins produced by the two L. plantarum strains and matrix- and strain-related differences in the susceptibility of L. monocytogenes to plantaricins and organic acids. In conclusion, the present study represents the first demonstration of the ability of a bacteriocin-producing LAB to dissipate the proton gradient of sessile and planktonic L. monocytogenes.

KW - Former LIFE faculty

KW - Listeria monocytogenes

KW - Bacteriocins

KW - FRIM

KW - Intracellular pH

U2 - 10.1016/j.ijfoodmicro.2010.03.040

DO - 10.1016/j.ijfoodmicro.2010.03.040

M3 - Journal article

C2 - 20447709

SN - 0168-1605

VL - 141

SP - S53-S59

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - Suppl. 1

ER -