¹H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat

TY - JOUR

T1 - 1H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat

AU - Tomassini, Alberta

AU - Vitalone, Annabella

AU - Marini, Federico

AU - Praticò, Giulia

AU - Sciubba, Fabio

AU - Bevilacqua, Marta

AU - Delfini, Maurizio

AU - Di Sotto, Antonella

AU - Di Giacomo, Silvia

AU - Mariani, Paola

AU - Mammola, Caterina L.

AU - Gaudio, Eugenio

AU - Miccheli, Alfredo

AU - Mazzanti, Gabriela

PY - 2014

Y1 - 2014

N2 - The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An 1H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by 1H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.

AB - The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An 1H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by 1H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.

KW - ASCA

KW - maternal separation

KW - metabolic profiling

KW - metabolomics

KW - NMR spectroscopy

KW - urine

U2 - 10.1021/pr500748r

DO - 10.1021/pr500748r

M3 - Journal article

C2 - 25299838

AN - SCOPUS:84915749793

SN - 1535-3893

VL - 13

SP - 5848

EP - 5859

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 12

ER -