1H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat

Alberta Tomassini; Annabella Vitalone; Federico Marini; Giulia Praticò; Fabio Sciubba; Marta Bevilacqua; Maurizio Delfini; Antonella Di Sotto; Silvia Di Giacomo; Paola Mariani; Caterina L. Mammola; Eugenio Gaudio; Alfredo Miccheli; Gabriela Mazzanti

doi:10.1021/pr500748r

¹H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat

Alberta Tomassini, Annabella Vitalone, Federico Marini, Giulia Praticò, Fabio Sciubba, Marta Bevilacqua, Maurizio Delfini, Antonella Di Sotto, Silvia Di Giacomo, Paola Mariani, Caterina L. Mammola, Eugenio Gaudio, Alfredo Miccheli^*, Gabriela Mazzanti

^*Corresponding author for this work

10 Citations (Scopus)

Abstract

The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An ¹H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by ¹H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.

Original language	English
Journal	Journal of Proteome Research
Volume	13
Issue number	12
Pages (from-to)	5848-5859
Number of pages	12
ISSN	1535-3893
DOIs	https://doi.org/10.1021/pr500748r
Publication status	Published - 2014
Externally published	Yes

Keywords

ASCA
maternal separation
metabolic profiling
metabolomics
NMR spectroscopy
urine

Access to Document

10.1021/pr500748r

Cite this

Tomassini, A., Vitalone, A., Marini, F., Praticò, G., Sciubba, F., Bevilacqua, M., Delfini, M., Di Sotto, A., Di Giacomo, S., Mariani, P., Mammola, C. L., Gaudio, E., Miccheli, A., & Mazzanti, G. (2014). ¹H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat. Journal of Proteome Research, 13(12), 5848-5859. https://doi.org/10.1021/pr500748r

Tomassini, A, Vitalone, A, Marini, F, Praticò, G, Sciubba, F, Bevilacqua, M, Delfini, M, Di Sotto, A, Di Giacomo, S, Mariani, P, Mammola, CL, Gaudio, E, Miccheli, A & Mazzanti, G 2014, '¹H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat', Journal of Proteome Research, vol. 13, no. 12, pp. 5848-5859. https://doi.org/10.1021/pr500748r

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title = "1H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat",

abstract = "The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An 1H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by 1H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.",

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author = "Alberta Tomassini and Annabella Vitalone and Federico Marini and Giulia Pratic{\`o} and Fabio Sciubba and Marta Bevilacqua and Maurizio Delfini and {Di Sotto}, Antonella and {Di Giacomo}, Silvia and Paola Mariani and Mammola, {Caterina L.} and Eugenio Gaudio and Alfredo Miccheli and Gabriela Mazzanti",

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T1 - 1H NMR-based urinary metabolic profiling reveals changes in nicotinamide pathway intermediates due to postnatal stress model in rat

AU - Tomassini, Alberta

AU - Vitalone, Annabella

AU - Marini, Federico

AU - Praticò, Giulia

AU - Sciubba, Fabio

AU - Bevilacqua, Marta

AU - Delfini, Maurizio

AU - Di Sotto, Antonella

AU - Di Giacomo, Silvia

AU - Mariani, Paola

AU - Mammola, Caterina L.

AU - Gaudio, Eugenio

AU - Miccheli, Alfredo

AU - Mazzanti, Gabriela

PY - 2014

Y1 - 2014

N2 - The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An 1H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by 1H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.

AB - The maternal separation protocol in rodents is a widely recognized model of early life stress allowing acute and chronic physiological consequences to be studied. An 1H NMR-based metabolomic approach was applied to urines to evaluate the systemic metabolic consequences of maternal separation stress in female rats after the beginning of weaning and 4 weeks later when the rats were reaching adulthood. Furthermore, because maternal separation is considered as a model mimicking the inflammatory bowel syndrome, the lactulose/mannitol test was used to evaluate the influence of postnatal maternal separation on gut permeability and mucosal barrier function by 1H NMR spectroscopy analysis of urine. The results showed no statistical differences in gut permeability due to maternal separation. The application of ANOVA simultaneous component analysis allowed the contributions of physiological adaptations to the animal's development to be separated from the metabolic consequences due to postnatal stress. Systemic metabolic differences in the maternally separated pups were mainly due to the tryptophan/NAD pathway intermediate levels and to the methyladenosine level. Urinary NMR-based metabolic profiling allowed us to disentangle the metabolic adaptive response of the rats to postnatal stress during the animal's growth, highlighting the metabolic changes induced by weaning, gut closure, and maturity.

KW - ASCA

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KW - metabolic profiling

KW - metabolomics

KW - NMR spectroscopy

KW - urine

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DO - 10.1021/pr500748r

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