Sensitivity to oxazolone induced dermatitis is transferable with gut microbiota in mice

TY - JOUR

T1 - Sensitivity to oxazolone induced dermatitis is transferable with gut microbiota in mice

AU - Fisker, Line

AU - Krych, Lukasz

AU - Engkilde, Kåre

AU - Nielsen, Dennis Sandris

AU - Kot, Witold Piotr

AU - Hansen, Camilla Hartmann Friis

AU - Hansen, Axel Kornerup

PY - 2017

Y1 - 2017

N2 - Atopic Dermatitis (AD) has been associated with gut microbiota (GM) dysbiosis in humans, indicating a causative role of GM in AD etiology. Furthermore, the GM strongly correlates to essential disease parameters in the well-known oxazolone-induced mouse model of AD. Here, we demonstrate that it is possible to transfer both a high-responding and a low-responding AD phenotype with GM from conventional mice to germ-free mice. The mice inoculated with the high-responding GM had significantly higher clinical score, increased ear thickness, and increased levels of IL-1β, TNFα, IL-4, IL-5, and IL-6 compared to the mice inoculated with the low-responding GM. The inter-individual variation was in general not affected by this increase in effect size. Germ-free mice induced with AD revealed a high disease response as well as high inter-individual variation indicating protective properties of certain microbial taxa in this model. This study underlines that the GM has a strong impact on AD in mouse models, and that the power of studies may be increased by the application of mice inoculated with a specific GM from high responders to increase the effect size.

AB - Atopic Dermatitis (AD) has been associated with gut microbiota (GM) dysbiosis in humans, indicating a causative role of GM in AD etiology. Furthermore, the GM strongly correlates to essential disease parameters in the well-known oxazolone-induced mouse model of AD. Here, we demonstrate that it is possible to transfer both a high-responding and a low-responding AD phenotype with GM from conventional mice to germ-free mice. The mice inoculated with the high-responding GM had significantly higher clinical score, increased ear thickness, and increased levels of IL-1β, TNFα, IL-4, IL-5, and IL-6 compared to the mice inoculated with the low-responding GM. The inter-individual variation was in general not affected by this increase in effect size. Germ-free mice induced with AD revealed a high disease response as well as high inter-individual variation indicating protective properties of certain microbial taxa in this model. This study underlines that the GM has a strong impact on AD in mouse models, and that the power of studies may be increased by the application of mice inoculated with a specific GM from high responders to increase the effect size.

U2 - 10.1038/srep44385

DO - 10.1038/srep44385

M3 - Journal article

C2 - 28290517

AN - SCOPUS:85015307682

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 44385

ER -