Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates

Jayda Lee Ann Siggers; Mette Viberg Østergaard; Richard Harvey Siggers; Kerstin Skovgaard; Lars Mølbak; Thomas Thymann; Mette Schmidt; Hanne Kristine Møller; Stig Purup; Lisbeth Nielsen Fink; Hanne Frøkiær; Mette Boye; Per Torp Sangild; Stine Brandt Bering

doi:10.1152/ajpgi.00278.2012

Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates

Jayda Lee Ann Siggers, Mette Viberg Østergaard, Richard Harvey Siggers, Kerstin Skovgaard, Lars Mølbak, Thomas Thymann, Mette Schmidt, Hanne Kristine Møller, Stig Purup, Lisbeth Nielsen Fink, Hanne Frøkiær, Mette Boye, Per Torp Sangild, Stine Brandt Bering

Institut for Idræt og Ernæring

52 Citationer (Scopus)

Abstract

Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, and antimicrobial factors. The fetal enteral diet amniotic fluid (AF), contains similar components, and we hypothesized that postnatal AF administration reduces inflammatory responses and NEC in preterm neonates. Preterm pigs (92% gestation) were delivered by caesarean section and fed parental nutrition (2 days) followed by enteral (2 days) porcine colostrum (COLOS, n = 7), infant formula (FORM, n = 13), or AF supplied before and after introduction of formula (AF, n = 10) in experiment 1, and supplied only during the enteral feeding period in experiment 2 (FORM, n = 16; AF, n = 14). The NEC score was reduced in both AF and COLOS pigs, relative to FORM, when AF was provided prior to full enteral feeding (9.9 and 7.7 compared with 17.3, P < 0.05). There was no effect of AF when provided only during enteral feeding. AF pigs showed decreased bacterial abundance in colon and intestinal inflammation-related genes (e.g., TNF-α, IL-1α, IL-6, NOS) were downregulated, relative to FORM pigs with NEC. Anti-inflammatory properties of AF were supported by delayed maturation and decreased TNF-α production in murine dendritic cells, as well as increased proliferation and migration, and downregulation of IL-6 expression in intestinal cells (IEC-6, IPEC-J2). Like colostrum, AF may reduce NEC development in preterm neonates by suppressing the proinflammatory responses to enteral formula feeding and gut colonization when provided before the onset of NEC.

Originalsprog	Engelsk
Tidsskrift	American Journal of Physiology: Gastrointestinal and Liver Physiology
Vol/bind	304
Udgave nummer	10
Sider (fra-til)	G864-G875
Antal sider	12
ISSN	0193-1857
DOI	https://doi.org/10.1152/ajpgi.00278.2012
Status	Udgivet - 2013

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10.1152/ajpgi.00278.2012

Citationsformater

Siggers, J. L. A., Østergaard, M. V., Siggers, R. H., Skovgaard, K., Mølbak, L., Thymann, T., Schmidt, M., Møller, H. K., Purup, S., Fink, L. N., Frøkiær, H., Boye, M., Sangild, P. T., & Bering, S. B. (2013). Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates. American Journal of Physiology: Gastrointestinal and Liver Physiology, 304(10), G864-G875. https://doi.org/10.1152/ajpgi.00278.2012

Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates. / Siggers, Jayda Lee Ann; Østergaard, Mette Viberg; Siggers, Richard Harvey et al.

I: American Journal of Physiology: Gastrointestinal and Liver Physiology, Bind 304, Nr. 10, 2013, s. G864-G875.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Siggers, JLA, Østergaard, MV, Siggers, RH, Skovgaard, K, Mølbak, L, Thymann, T, Schmidt, M, Møller, HK, Purup, S, Fink, LN, Frøkiær, H, Boye, M, Sangild, PT & Bering, SB 2013, 'Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates', American Journal of Physiology: Gastrointestinal and Liver Physiology, bind 304, nr. 10, s. G864-G875. https://doi.org/10.1152/ajpgi.00278.2012

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abstract = "Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, and antimicrobial factors. The fetal enteral diet amniotic fluid (AF), contains similar components, and we hypothesized that postnatal AF administration reduces inflammatory responses and NEC in preterm neonates. Preterm pigs (92% gestation) were delivered by caesarean section and fed parental nutrition (2 days) followed by enteral (2 days) porcine colostrum (COLOS, n = 7), infant formula (FORM, n = 13), or AF supplied before and after introduction of formula (AF, n = 10) in experiment 1, and supplied only during the enteral feeding period in experiment 2 (FORM, n = 16; AF, n = 14). The NEC score was reduced in both AF and COLOS pigs, relative to FORM, when AF was provided prior to full enteral feeding (9.9 and 7.7 compared with 17.3, P < 0.05). There was no effect of AF when provided only during enteral feeding. AF pigs showed decreased bacterial abundance in colon and intestinal inflammation-related genes (e.g., TNF-α, IL-1α, IL-6, NOS) were downregulated, relative to FORM pigs with NEC. Anti-inflammatory properties of AF were supported by delayed maturation and decreased TNF-α production in murine dendritic cells, as well as increased proliferation and migration, and downregulation of IL-6 expression in intestinal cells (IEC-6, IPEC-J2). Like colostrum, AF may reduce NEC development in preterm neonates by suppressing the proinflammatory responses to enteral formula feeding and gut colonization when provided before the onset of NEC.",

author = "Siggers, {Jayda Lee Ann} and {\O}stergaard, {Mette Viberg} and Siggers, {Richard Harvey} and Kerstin Skovgaard and Lars M{\o}lbak and Thomas Thymann and Mette Schmidt and M{\o}ller, {Hanne Kristine} and Stig Purup and Fink, {Lisbeth Nielsen} and Hanne Fr{\o}ki{\ae}r and Mette Boye and Sangild, {Per Torp} and Bering, {Stine Brandt}",

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T1 - Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates

AU - Siggers, Jayda Lee Ann

AU - Østergaard, Mette Viberg

AU - Siggers, Richard Harvey

AU - Skovgaard, Kerstin

AU - Mølbak, Lars

AU - Thymann, Thomas

AU - Schmidt, Mette

AU - Møller, Hanne Kristine

AU - Purup, Stig

AU - Fink, Lisbeth Nielsen

AU - Frøkiær, Hanne

AU - Boye, Mette

AU - Sangild, Per Torp

AU - Bering, Stine Brandt

N1 - CURIS 2013 NEXS 117

PY - 2013

Y1 - 2013

N2 - Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, and antimicrobial factors. The fetal enteral diet amniotic fluid (AF), contains similar components, and we hypothesized that postnatal AF administration reduces inflammatory responses and NEC in preterm neonates. Preterm pigs (92% gestation) were delivered by caesarean section and fed parental nutrition (2 days) followed by enteral (2 days) porcine colostrum (COLOS, n = 7), infant formula (FORM, n = 13), or AF supplied before and after introduction of formula (AF, n = 10) in experiment 1, and supplied only during the enteral feeding period in experiment 2 (FORM, n = 16; AF, n = 14). The NEC score was reduced in both AF and COLOS pigs, relative to FORM, when AF was provided prior to full enteral feeding (9.9 and 7.7 compared with 17.3, P < 0.05). There was no effect of AF when provided only during enteral feeding. AF pigs showed decreased bacterial abundance in colon and intestinal inflammation-related genes (e.g., TNF-α, IL-1α, IL-6, NOS) were downregulated, relative to FORM pigs with NEC. Anti-inflammatory properties of AF were supported by delayed maturation and decreased TNF-α production in murine dendritic cells, as well as increased proliferation and migration, and downregulation of IL-6 expression in intestinal cells (IEC-6, IPEC-J2). Like colostrum, AF may reduce NEC development in preterm neonates by suppressing the proinflammatory responses to enteral formula feeding and gut colonization when provided before the onset of NEC.

AB - Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, and antimicrobial factors. The fetal enteral diet amniotic fluid (AF), contains similar components, and we hypothesized that postnatal AF administration reduces inflammatory responses and NEC in preterm neonates. Preterm pigs (92% gestation) were delivered by caesarean section and fed parental nutrition (2 days) followed by enteral (2 days) porcine colostrum (COLOS, n = 7), infant formula (FORM, n = 13), or AF supplied before and after introduction of formula (AF, n = 10) in experiment 1, and supplied only during the enteral feeding period in experiment 2 (FORM, n = 16; AF, n = 14). The NEC score was reduced in both AF and COLOS pigs, relative to FORM, when AF was provided prior to full enteral feeding (9.9 and 7.7 compared with 17.3, P < 0.05). There was no effect of AF when provided only during enteral feeding. AF pigs showed decreased bacterial abundance in colon and intestinal inflammation-related genes (e.g., TNF-α, IL-1α, IL-6, NOS) were downregulated, relative to FORM pigs with NEC. Anti-inflammatory properties of AF were supported by delayed maturation and decreased TNF-α production in murine dendritic cells, as well as increased proliferation and migration, and downregulation of IL-6 expression in intestinal cells (IEC-6, IPEC-J2). Like colostrum, AF may reduce NEC development in preterm neonates by suppressing the proinflammatory responses to enteral formula feeding and gut colonization when provided before the onset of NEC.

U2 - 10.1152/ajpgi.00278.2012

DO - 10.1152/ajpgi.00278.2012

M3 - Journal article

C2 - 23518680

SN - 0193-1857

VL - 304

SP - G864-G875

JO - American Journal of Physiology: Gastrointestinal and Liver Physiology

JF - American Journal of Physiology: Gastrointestinal and Liver Physiology

IS - 10

ER -

Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates

Abstract

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