Plasma membrane Ca²⁺ transporters mediate virus-induced acquired resistance to oxidative stress

TY - JOUR

T1 - Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress

AU - Shabala, Sergey

AU - Bækgaard, Lone

AU - Shabala, Lana

AU - Fuglsang, Anja Thoe

AU - Babourina, Olga

AU - Palmgren, Michael Broberg

AU - Cuin, Tracey A.

AU - Rengel, Zed

AU - Nemchinov, Lev G.

PY - 2011/3

Y1 - 2011/3

N2 - This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca2+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H2O2] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca2+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca2+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca2+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca2+ efflux systems in acquired resistance to oxidative stress in plants.

AB - This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca2+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H2O2] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca2+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca2+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca2+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca2+ efflux systems in acquired resistance to oxidative stress in plants.

U2 - 10.1111/j.1365-3040.2010.02251.x

DO - 10.1111/j.1365-3040.2010.02251.x

M3 - Journal article

C2 - 21062316

SN - 0140-7791

VL - 34

SP - 406

EP - 417

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 3

ER -