Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress

Sergey Shabala; Lone Bækgaard; Lana Shabala; Anja Thoe Fuglsang; Olga Babourina; Michael Broberg Palmgren; Tracey A. Cuin; Zed Rengel; Lev G. Nemchinov

doi:10.1111/j.1365-3040.2010.02251.x

Plasma membrane Ca²⁺ transporters mediate virus-induced acquired resistance to oxidative stress

Sergey Shabala, Lone Bækgaard, Lana Shabala, Anja Thoe Fuglsang, Olga Babourina, Michael Broberg Palmgren, Tracey A. Cuin, Zed Rengel, Lev G. Nemchinov

34 Citations (Scopus)

Abstract

This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca²⁺ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H₂O₂] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca²⁺- and H⁺-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca²⁺ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca²⁺ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca²⁺ efflux systems in acquired resistance to oxidative stress in plants.

Original language	English
Journal	Plant, Cell and Environment
Volume	34
Issue number	3
Pages (from-to)	406-417
Number of pages	12
ISSN	0140-7791
DOIs	https://doi.org/10.1111/j.1365-3040.2010.02251.x
Publication status	Published - Mar 2011

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title = "Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress",

abstract = "This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca2+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H2O2] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca2+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca2+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca2+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca2+ efflux systems in acquired resistance to oxidative stress in plants.",

author = "Sergey Shabala and Lone B{\ae}kgaard and Lana Shabala and Fuglsang, {Anja Thoe} and Olga Babourina and Palmgren, {Michael Broberg} and Cuin, {Tracey A.} and Zed Rengel and Nemchinov, {Lev G.}",

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language = "English",

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T1 - Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress

AU - Shabala, Sergey

AU - Bækgaard, Lone

AU - Shabala, Lana

AU - Fuglsang, Anja Thoe

AU - Babourina, Olga

AU - Palmgren, Michael Broberg

AU - Cuin, Tracey A.

AU - Rengel, Zed

AU - Nemchinov, Lev G.

PY - 2011/3

Y1 - 2011/3

N2 - This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca2+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H2O2] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca2+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca2+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca2+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca2+ efflux systems in acquired resistance to oxidative stress in plants.

AB - This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca2+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H2O2] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca2+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca2+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca2+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca2+ efflux systems in acquired resistance to oxidative stress in plants.

U2 - 10.1111/j.1365-3040.2010.02251.x

DO - 10.1111/j.1365-3040.2010.02251.x

M3 - Journal article

C2 - 21062316

SN - 0140-7791

VL - 34

SP - 406

EP - 417

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 3

ER -

Plasma membrane Ca2+ transporters mediate virus-induced acquired resistance to oxidative stress

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Plasma membrane Ca²⁺ transporters mediate virus-induced acquired resistance to oxidative stress