Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Alexey Brazhe; Nadezda Brazhe; Georgy V Maksimov; Pavel S Ignatyev; Andrey B Rubin; Erik Mosekilde; Olga Sosnovtseva

doi:10.1117/1.2937213

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Alexey Brazhe, Nadezda Brazhe, Georgy V Maksimov, Pavel S Ignatyev, Andrey B Rubin, Erik Mosekilde, Olga Sosnovtseva

Nyre- og Karforskning

15 Citationer (Scopus)

Abstract

We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

Originalsprog	Engelsk
Tidsskrift	Journal of Biomedical Optics
Vol/bind	13
Udgave nummer	3
Sider (fra-til)	034004
ISSN	1083-3668
DOI	https://doi.org/10.1117/1.2937213
Status	Udgivet - 2008

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10.1117/1.2937213

Citationsformater

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title = "Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study",

abstract = "We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.",

keywords = "Cells, Cultured, Erythrocytes, Humans, Image Enhancement, Interferometry, Microscopy, Confocal, Microscopy, Phase-Contrast, Tomography, Optical Coherence",

author = "Alexey Brazhe and Nadezda Brazhe and Maksimov, {Georgy V} and Ignatyev, {Pavel S} and Rubin, {Andrey B} and Erik Mosekilde and Olga Sosnovtseva",

year = "2008",

doi = "10.1117/1.2937213",

language = "English",

volume = "13",

pages = "034004",

journal = "Journal of Biomedical Optics",

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T1 - Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

AU - Brazhe, Alexey

AU - Brazhe, Nadezda

AU - Maksimov, Georgy V

AU - Ignatyev, Pavel S

AU - Rubin, Andrey B

AU - Mosekilde, Erik

AU - Sosnovtseva, Olga

PY - 2008

Y1 - 2008

N2 - We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

AB - We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

KW - Cells, Cultured

KW - Erythrocytes

KW - Humans

KW - Image Enhancement

KW - Interferometry

KW - Microscopy, Confocal

KW - Microscopy, Phase-Contrast

KW - Tomography, Optical Coherence

U2 - 10.1117/1.2937213

DO - 10.1117/1.2937213

M3 - Journal article

C2 - 18601549

SN - 1083-3668

VL - 13

SP - 034004

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 3

ER -

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

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