Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Alexey Brazhe; Nadezda Brazhe; Georgy V Maksimov; Pavel S Ignatyev; Andrey B Rubin; Erik Mosekilde; Olga Sosnovtseva

doi:10.1117/1.2937213

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Alexey Brazhe, Nadezda Brazhe, Georgy V Maksimov, Pavel S Ignatyev, Andrey B Rubin, Erik Mosekilde, Olga Sosnovtseva

Renal and Vascular Research

15 Citations (Scopus)

Abstract

We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

Original language	English
Journal	Journal of Biomedical Optics
Volume	13
Issue number	3
Pages (from-to)	034004
ISSN	1083-3668
DOIs	https://doi.org/10.1117/1.2937213
Publication status	Published - 2008

Keywords

Cells, Cultured
Erythrocytes
Humans
Image Enhancement
Interferometry
Microscopy, Confocal
Microscopy, Phase-Contrast
Tomography, Optical Coherence

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10.1117/1.2937213

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title = "Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study",

abstract = "We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.",

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doi = "10.1117/1.2937213",

language = "English",

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T1 - Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

AU - Brazhe, Alexey

AU - Brazhe, Nadezda

AU - Maksimov, Georgy V

AU - Ignatyev, Pavel S

AU - Rubin, Andrey B

AU - Mosekilde, Erik

AU - Sosnovtseva, Olga

PY - 2008

Y1 - 2008

N2 - We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

AB - We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.

KW - Cells, Cultured

KW - Erythrocytes

KW - Humans

KW - Image Enhancement

KW - Interferometry

KW - Microscopy, Confocal

KW - Microscopy, Phase-Contrast

KW - Tomography, Optical Coherence

U2 - 10.1117/1.2937213

DO - 10.1117/1.2937213

M3 - Journal article

C2 - 18601549

SN - 1083-3668

VL - 13

SP - 034004

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 3

ER -

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Abstract

Keywords

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