Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release

Nilofar V. Følsgaard; Susanne Schjorring; Bo L. Chawes; Morten Arendt Rasmussen; Karen A. Krogfelt; Susanne Brix Pedersen; Hans Bisgaard

doi:10.1164/rccm.201207-1297oc

Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release

Nilofar V. Følsgaard, Susanne Schjorring, Bo L. Chawes, Morten Arendt Rasmussen, Karen A. Krogfelt, Susanne Brix Pedersen, Hans Bisgaard

89 Citationer (Scopus)

Abstract

Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhal's, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1 beta (M. catarrhalis, P = 2.2 x 10(-12); H. influenzae, P = 7.1 X 10(-10)), TNF-alpha (M. catarrhalis, P = 1.5 x 10(-9); H. influenzae, P = 5.9 x 10(-7)), and macrophage inflammatory protein-1 beta (M. catarrhalis, P = 1.6 X 10(-11); H. influenzae, P = 2.7 x 10(-7)). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 x 10(-24)). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.

Originalsprog	Engelsk
Tidsskrift	American Journal of Respiratory and Critical Care Medicine
Vol/bind	187
Udgave nummer	6
Sider (fra-til)	589-595
Antal sider	7
ISSN	1073-449X
DOI	https://doi.org/10.1164/rccm.201207-1297oc
Status	Udgivet - 15 mar. 2013

Emneord

bacterial colonization
airways
neonatal
cytokine
chemokine

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10.1164/rccm.201207-1297oc

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Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release. / Følsgaard, Nilofar V.; Schjorring, Susanne; Chawes, Bo L. et al.

I: American Journal of Respiratory and Critical Care Medicine, Bind 187, Nr. 6, 15.03.2013, s. 589-595.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{8b234d3c38224682aa6877412cb91725,

title = "Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release",

abstract = "Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1β (M. catarrhalis, P = 2.2 × 10-12; H. influenzae, P = 7.1 × 10-10), TNF-α (M. catarrhalis, P = 1.5 × 10-9; H. influenzae,P=5.9×10-7),andmacrophageinflammatoryprotein- 1β (M. catarrhalis, P = 1.6 × 10-11; H. influenzae, P = 2.7 × 10-7). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 × 10 -24). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.",

keywords = "bacterial colonization, airways, neonatal, cytokine, chemokine",

author = "F{\o}lsgaard, {Nilofar V.} and Susanne Schjorring and Chawes, {Bo L.} and Rasmussen, {Morten Arendt} and Krogfelt, {Karen A.} and Pedersen, {Susanne Brix} and Hans Bisgaard",

year = "2013",

month = mar,

day = "15",

doi = "10.1164/rccm.201207-1297oc",

language = "English",

volume = "187",

pages = "589--595",

journal = "American Journal of Respiratory and Critical Care Medicine",

issn = "1073-449X",

publisher = "American Thoracic Society",

number = "6",

}

TY - JOUR

T1 - Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release

AU - Følsgaard, Nilofar V.

AU - Schjorring, Susanne

AU - Chawes, Bo L.

AU - Rasmussen, Morten Arendt

AU - Krogfelt, Karen A.

AU - Pedersen, Susanne Brix

AU - Bisgaard, Hans

PY - 2013/3/15

Y1 - 2013/3/15

N2 - Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1β (M. catarrhalis, P = 2.2 × 10-12; H. influenzae, P = 7.1 × 10-10), TNF-α (M. catarrhalis, P = 1.5 × 10-9; H. influenzae,P=5.9×10-7),andmacrophageinflammatoryprotein- 1β (M. catarrhalis, P = 1.6 × 10-11; H. influenzae, P = 2.7 × 10-7). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 × 10 -24). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.

AB - Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1β (M. catarrhalis, P = 2.2 × 10-12; H. influenzae, P = 7.1 × 10-10), TNF-α (M. catarrhalis, P = 1.5 × 10-9; H. influenzae,P=5.9×10-7),andmacrophageinflammatoryprotein- 1β (M. catarrhalis, P = 1.6 × 10-11; H. influenzae, P = 2.7 × 10-7). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 × 10 -24). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.

KW - bacterial colonization

KW - airways

KW - neonatal

KW - cytokine

KW - chemokine

U2 - 10.1164/rccm.201207-1297oc

DO - 10.1164/rccm.201207-1297oc

M3 - Journal article

C2 - 23370914

SN - 1073-449X

VL - 187

SP - 589

EP - 595

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

IS - 6

ER -

Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release

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