TY - JOUR
T1 - Pathogenic bacteria colonizing the airways in asymptomatic neonates stimulates topical inflammatory mediator release
AU - Følsgaard, Nilofar V.
AU - Schjorring, Susanne
AU - Chawes, Bo L.
AU - Rasmussen, Morten Arendt
AU - Krogfelt, Karen A.
AU - Pedersen, Susanne Brix
AU - Bisgaard, Hans
PY - 2013/3/15
Y1 - 2013/3/15
N2 - Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1β (M. catarrhalis, P = 2.2 × 10-12; H. influenzae, P = 7.1 × 10-10), TNF-α (M. catarrhalis, P = 1.5 × 10-9; H. influenzae,P=5.9×10-7),andmacrophageinflammatoryprotein- 1β (M. catarrhalis, P = 1.6 × 10-11; H. influenzae, P = 2.7 × 10-7). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 × 10 -24). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.
AB - Rationale: Bacterial colonization of neonatal airways with the pathogenic bacterial species, Moraxella catarrhalis, Streptococcus pneumoniae, and Haemophilus influenzae, is associated with later development of childhood asthma. Objectives: To study a possible association between colonization with pathogenic bacterial strains and the immune signature of the upper airways in healthy neonates. Methods: A total of 20 cytokines and chemokines were quantified in vivo in the airway mucosal lining fluid of 662 neonates from the Copenhagen Prospective Study of Asthma in Childhood 2010 birth cohort. Colonization of the hypopharynx with M. catarrhalis, S. pneumoniae, H. influenzae, and Staphylococcus aureus was assessed simultaneously. The association between immune signatures and bacterial colonization or noncolonized controls was analyzed using conventional statistical methods supplemented by a multivariate approach for pattern identification. Measurements and Main Results: Colonization with M. catarrhalis and H. influenzae induced a mixed T helper cell (Th) type 1/Th2/Th17 response with high levels of IL-1β (M. catarrhalis, P = 2.2 × 10-12; H. influenzae, P = 7.1 × 10-10), TNF-α (M. catarrhalis, P = 1.5 × 10-9; H. influenzae,P=5.9×10-7),andmacrophageinflammatoryprotein- 1β (M. catarrhalis, P = 1.6 × 10-11; H. influenzae, P = 2.7 × 10-7). S. aureus colonization demonstrated a Th17-promoting profile with elevated IL-17 levels (P = 1.6 × 10 -24). S. pneumoniae colonization was not significantly associated with any of the mediators. Conclusions: M. catarrhalis and H. influenzae colonization of the airways of asymptomatic neonates is associated with an inflammatory immune response of the airway mucosa, which may result in chronic inflammation.
KW - bacterial colonization
KW - airways
KW - neonatal
KW - cytokine
KW - chemokine
U2 - 10.1164/rccm.201207-1297oc
DO - 10.1164/rccm.201207-1297oc
M3 - Journal article
C2 - 23370914
SN - 1073-449X
VL - 187
SP - 589
EP - 595
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
IS - 6
ER -