TY - JOUR
T1 - Parallel artificial liquid membrane extraction of psychoactive analytes: a novel approach in therapeutic drug monitoring
AU - Olsen, Katharina Norgren
AU - Ask, Kristine Skoglund
AU - Pedersen-Bjergaard, Stig
AU - Gjelstad, Astrid
PY - 2018/3
Y1 - 2018/3
N2 - Aim: Liquid-liquid extraction is widely used in therapeutic drug monitoring of antipsychotics, but difficulties in automation of the technique can result in long operational time. In this paper, parallel artificial liquid membrane extraction was used for extraction of serotonin- and serotonin-norepinephrine reuptake inhibitors from human plasma, and an approach to automate the technique was investigated. Results: Eight model analytes were extracted from 125 μl human plasma with recoveries in the range 72-111% (relative standard deviation [RSD] ≤12.8%). A semiautomated pipettor was successfully utilized in the procedure, reducing the manual handling time. Real patient samples were analyzed with satisfying accuracy. Conclusion: A semiautomated extraction of serotonin-and serotonin-norepinephrine reuptake inhibitors by parallel artificial liquid membrane extraction extraction was successfully performed.
AB - Aim: Liquid-liquid extraction is widely used in therapeutic drug monitoring of antipsychotics, but difficulties in automation of the technique can result in long operational time. In this paper, parallel artificial liquid membrane extraction was used for extraction of serotonin- and serotonin-norepinephrine reuptake inhibitors from human plasma, and an approach to automate the technique was investigated. Results: Eight model analytes were extracted from 125 μl human plasma with recoveries in the range 72-111% (relative standard deviation [RSD] ≤12.8%). A semiautomated pipettor was successfully utilized in the procedure, reducing the manual handling time. Real patient samples were analyzed with satisfying accuracy. Conclusion: A semiautomated extraction of serotonin-and serotonin-norepinephrine reuptake inhibitors by parallel artificial liquid membrane extraction extraction was successfully performed.
U2 - 10.4155/bio-2017-0250
DO - 10.4155/bio-2017-0250
M3 - Journal article
C2 - 29451001
SN - 1757-6180
VL - 10
SP - 385
EP - 395
JO - Bioanalysis
JF - Bioanalysis
ER -