Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

TY - GEN

T1 - Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

AU - Glückstad, Jesper

AU - Rodrigo, Peter J.

AU - Daria, Vincent R.

AU - Siegumfeldt, Henrik

AU - Nissen, Peter

AU - Arneborg, Nils

PY - 2005/7/21

Y1 - 2005/7/21

N2 - We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

AB - We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

KW - Generalized phase contrast

KW - Manipulation of living cells

KW - Real-time multi-beam optical trapping

UR - http://www.scopus.com/inward/record.url?scp=21844465640&partnerID=8YFLogxK

U2 - 10.1117/12.590264

DO - 10.1117/12.590264

M3 - Conference article

AN - SCOPUS:21844465640

SN - 1605-7422

VL - 5699

SP - 306

EP - 312

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 46

T2 - Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

Y2 - 24 January 2005 through 27 January 2005

ER -