Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates

Tanja Larsen, Yayoi Yoshimura, Bjørn G. R. Voldborg, Giuseppe Cazzamali, Nicolai V. Bovin, Ulrika Westerlind, Monica M. Palcic, Jørgen Leisner

7 Citationer (Scopus)

Abstract

Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher's disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugates as alternate substrates. LacNAc and LacdiNAc-terminating substrates are hydrolyzed, the latter with a turnover comparable to that of pNP-chitotriose. Glycolipids or glycoproteins with LacNAc and LacdiNAc represent potential chitinase substrates and the subsequent alteration of glycosylation pattern could be a factor in disease pathogenesis.

OriginalsprogEngelsk
TidsskriftF E B S Letters
Vol/bind588
Udgave nummer5
Sider (fra-til)746-751
Antal sider6
ISSN0014-5793
DOI
StatusUdgivet - 3 mar. 2014

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