Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates

Tanja Larsen; Yayoi Yoshimura; Bjørn G. R. Voldborg; Giuseppe Cazzamali; Nicolai V. Bovin; Ulrika Westerlind; Monica M. Palcic; Jørgen Leisner

doi:10.1016/j.febslet.2013.12.035

Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates

Tanja Larsen, Yayoi Yoshimura, Bjørn G. R. Voldborg, Giuseppe Cazzamali, Nicolai V. Bovin, Ulrika Westerlind, Monica M. Palcic, Jørgen Leisner

7 Citations (Scopus)

Abstract

Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher's disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugates as alternate substrates. LacNAc and LacdiNAc-terminating substrates are hydrolyzed, the latter with a turnover comparable to that of pNP-chitotriose. Glycolipids or glycoproteins with LacNAc and LacdiNAc represent potential chitinase substrates and the subsequent alteration of glycosylation pattern could be a factor in disease pathogenesis.

Original language	English
Journal	F E B S Letters
Volume	588
Issue number	5
Pages (from-to)	746-751
Number of pages	6
ISSN	0014-5793
DOIs	https://doi.org/10.1016/j.febslet.2013.12.035
Publication status	Published - 3 Mar 2014

Keywords

Faculty of Health and Medical Sciences
Chitinases
Family 18 glycosidases
Kinetics
GlcNAc-containing substrates
Glycoconjugates
LacNAc
LacdiNac

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10.1016/j.febslet.2013.12.035

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title = "Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates",

abstract = "Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher's disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugates as alternate substrates. LacNAc and LacdiNAc-terminating substrates are hydrolyzed, the latter with a turnover comparable to that of pNP-chitotriose. Glycolipids or glycoproteins with LacNAc and LacdiNAc represent potential chitinase substrates and the subsequent alteration of glycosylation pattern could be a factor in disease pathogenesis.",

keywords = "Faculty of Health and Medical Sciences, Chitinases, Family 18 glycosidases, Kinetics, GlcNAc-containing substrates, Glycoconjugates, LacNAc, LacdiNac",

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doi = "10.1016/j.febslet.2013.12.035",

language = "English",

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T1 - Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates

AU - Larsen, Tanja

AU - Yoshimura, Yayoi

AU - Voldborg, Bjørn G. R.

AU - Cazzamali, Giuseppe

AU - Bovin, Nicolai V.

AU - Westerlind, Ulrika

AU - Palcic, Monica M.

AU - Leisner, Jørgen

PY - 2014/3/3

Y1 - 2014/3/3

N2 - Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher's disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugates as alternate substrates. LacNAc and LacdiNAc-terminating substrates are hydrolyzed, the latter with a turnover comparable to that of pNP-chitotriose. Glycolipids or glycoproteins with LacNAc and LacdiNAc represent potential chitinase substrates and the subsequent alteration of glycosylation pattern could be a factor in disease pathogenesis.

AB - Humans do not synthesize chitin, yet they produce a number of active and inactive chitinases. One of the active enzymes is chitotriosidase whose serum levels are elevated in a number of diseases such as Gaucher's disease and upon fungal infection. Since the biological role of chitotriosidase in disease pathogenesis is not understood we screened a panel of mammalian GlcNAc-containing glycoconjugates as alternate substrates. LacNAc and LacdiNAc-terminating substrates are hydrolyzed, the latter with a turnover comparable to that of pNP-chitotriose. Glycolipids or glycoproteins with LacNAc and LacdiNAc represent potential chitinase substrates and the subsequent alteration of glycosylation pattern could be a factor in disease pathogenesis.

KW - Faculty of Health and Medical Sciences

KW - Chitinases

KW - Family 18 glycosidases

KW - Kinetics

KW - GlcNAc-containing substrates

KW - Glycoconjugates

KW - LacNAc

KW - LacdiNac

U2 - 10.1016/j.febslet.2013.12.035

DO - 10.1016/j.febslet.2013.12.035

M3 - Journal article

C2 - 24462685

SN - 0014-5793

VL - 588

SP - 746

EP - 751

JO - F E B S Letters

JF - F E B S Letters

IS - 5

ER -

Human chitotriosidase CHIT1 cross reacts with mammalian-like substrates

Abstract

Keywords

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