Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Johan Andersen-Ranberg; Kenneth Thermann Kongstad; Morten Thrane Nielsen; Niels Bjerg Jensen; Irini Pateraki; Søren Spanner Bach; Britta Hamberger; Philipp Zerbe; Dan Stærk; Jörg Bohlmann; Birger Lindberg Møller; Björn Robert Hamberger

doi:10.1002/anie.201510650

Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Johan Andersen-Ranberg, Kenneth Thermann Kongstad, Morten Thrane Nielsen, Niels Bjerg Jensen, Irini Pateraki, Søren Spanner Bach, Britta Hamberger, Philipp Zerbe, Dan Stærk, Jörg Bohlmann, Birger Lindberg Møller, Björn Robert Hamberger

Plantebiokemi

94 Citationer (Scopus)

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Abstract

Plant-derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are "new-to-nature". Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.

Originalsprog	Engelsk
Tidsskrift	Angewandte Chemie International Edition
Vol/bind	55
Udgave nummer	6
Sider (fra-til)	2142-2146
Antal sider	5
ISSN	1433-7851
DOI	https://doi.org/10.1002/anie.201510650
Status	Udgivet - 5 feb. 2016

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10.1002/anie.201510650

Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial BiosynthesisForlagets udgivne version, 4,14 MB

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Andersen-Ranberg, J., Kongstad, K. T., Nielsen, M. T., Bjerg Jensen, N., Pateraki, I., Bach, S. S., Hamberger, B., Zerbe, P., Stærk, D., Bohlmann, J., Møller, B. L., & Hamberger, B. R. (2016). Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis. Angewandte Chemie International Edition, 55(6), 2142-2146. https://doi.org/10.1002/anie.201510650

Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis. / Andersen-Ranberg, Johan ; Kongstad, Kenneth Thermann; Nielsen, Morten Thrane et al.
I: Angewandte Chemie International Edition, Bind 55, Nr. 6, 05.02.2016, s. 2142-2146.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Andersen-Ranberg, J , Kongstad, KT, Nielsen, MT, Bjerg Jensen, N, Pateraki, I, Bach, SS, Hamberger, B, Zerbe, P, Stærk, D, Bohlmann, J, Møller, BL & Hamberger, BR 2016, 'Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis', Angewandte Chemie International Edition, bind 55, nr. 6, s. 2142-2146. https://doi.org/10.1002/anie.201510650

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AU - Pateraki, Irini

AU - Bach, Søren Spanner

AU - Hamberger, Britta

AU - Zerbe, Philipp

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AU - Bohlmann, Jörg

AU - Møller, Birger Lindberg

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Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Abstract

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