Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Johan Andersen-Ranberg; Kenneth Thermann Kongstad; Morten Thrane Nielsen; Niels Bjerg Jensen; Irini Pateraki; Søren Spanner Bach; Britta Hamberger; Philipp Zerbe; Dan Stærk; Jörg Bohlmann; Birger Lindberg Møller; Björn Robert Hamberger

doi:10.1002/anie.201510650

Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Johan Andersen-Ranberg, Kenneth Thermann Kongstad, Morten Thrane Nielsen, Niels Bjerg Jensen, Irini Pateraki, Søren Spanner Bach, Britta Hamberger, Philipp Zerbe, Dan Stærk, Jörg Bohlmann, Birger Lindberg Møller, Björn Robert Hamberger

Section for Plant Biochemistry

94 Citations (Scopus)

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Abstract

Plant-derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are "new-to-nature". Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae.

Original language	English
Journal	Angewandte Chemie International Edition
Volume	55
Issue number	6
Pages (from-to)	2142-2146
Number of pages	5
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201510650
Publication status	Published - 5 Feb 2016

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Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial BiosynthesisFinal published version, 4.14 MB

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Andersen-Ranberg, J., Kongstad, K. T., Nielsen, M. T., Bjerg Jensen, N., Pateraki, I., Bach, S. S., Hamberger, B., Zerbe, P., Stærk, D., Bohlmann, J., Møller, B. L., & Hamberger, B. R. (2016). Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis. Angewandte Chemie International Edition, 55(6), 2142-2146. https://doi.org/10.1002/anie.201510650

Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis. / Andersen-Ranberg, Johan ; Kongstad, Kenneth Thermann; Nielsen, Morten Thrane et al.

In: Angewandte Chemie International Edition, Vol. 55, No. 6, 05.02.2016, p. 2142-2146.

Research output: Contribution to journal › Journal article › Research › peer-review

Andersen-Ranberg, J , Kongstad, KT, Nielsen, MT, Bjerg Jensen, N, Pateraki, I, Bach, SS, Hamberger, B, Zerbe, P, Stærk, D, Bohlmann, J, Møller, BL & Hamberger, BR 2016, 'Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis', Angewandte Chemie International Edition, vol. 55, no. 6, pp. 2142-2146. https://doi.org/10.1002/anie.201510650

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AU - Pateraki, Irini

AU - Bach, Søren Spanner

AU - Hamberger, Britta

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Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

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