Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA

TY - JOUR

T1 - Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA

AU - Dueholm, K L

AU - Buchardt, O

AU - Coull, J

AU - Nielsen, Peter E.

PY - 1995/1/25

Y1 - 1995/1/25

N2 - The synthesis and DNA binding properties of bis-PNA (peptide nucleic acid) are reported. Two PNA segments each of seven nucleobases in length were connected in a continuous synthesis via a flexible linker composed of three 8-amino-3,6-dioxaoctanoic acid units. The sequence of the first strand was TCTCTTT (C- to N-terminal), while the second strand was TTTCTCT or TTTJTJT, where J is pseudoisocytosine. These bis-PNAs form triple-stranded complexes of somewhat higher thermal stability than monomeric PNA with complementary oligonucleotides and the thermal melting transition shows very little hysteresis. When the J base is placed in the strand parallel to the DNA complement ('Hoogsteen strand'), the DNA binding was pH independent. The bis-PNAs were also superior to monomeric PNAs for targeting double-stranded DNA by strand invasion.

AB - The synthesis and DNA binding properties of bis-PNA (peptide nucleic acid) are reported. Two PNA segments each of seven nucleobases in length were connected in a continuous synthesis via a flexible linker composed of three 8-amino-3,6-dioxaoctanoic acid units. The sequence of the first strand was TCTCTTT (C- to N-terminal), while the second strand was TTTCTCT or TTTJTJT, where J is pseudoisocytosine. These bis-PNAs form triple-stranded complexes of somewhat higher thermal stability than monomeric PNA with complementary oligonucleotides and the thermal melting transition shows very little hysteresis. When the J base is placed in the strand parallel to the DNA complement ('Hoogsteen strand'), the DNA binding was pH independent. The bis-PNAs were also superior to monomeric PNAs for targeting double-stranded DNA by strand invasion.

KW - Base Sequence

KW - Binding Sites

KW - Cytosine/analogs & derivatives

KW - DNA/metabolism

KW - Drug Stability

KW - Hot Temperature

KW - Hydrogen-Ion Concentration

KW - Macromolecular Substances

KW - Molecular Sequence Data

KW - Molecular Structure

KW - Potassium Permanganate

KW - Structure-Activity Relationship

M3 - Journal article

C2 - 7862524

SN - 0305-1048

VL - 23

SP - 217

EP - 222

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 2

ER -