Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

Victor Abrahamsson; Signe Hoff; Nikoline Juul Nielsen; Marianne Lund Lametsch; Mogens Larsen Andersen

doi:10.1094/asbcj-2012-1012-01

Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

Victor Abrahamsson, Signe Hoff, Nikoline Juul Nielsen, Marianne Lund Lametsch, Mogens Larsen Andersen

19 Citationer (Scopus)

Abstract

A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

Originalsprog	Engelsk
Tidsskrift	Journal of the American Society of Brewing Chemists
Vol/bind	70
Udgave nummer	4
Sider (fra-til)	296-302
Antal sider	7
ISSN	0361-0470
DOI	https://doi.org/10.1094/asbcj-2012-1012-01
Status	Udgivet - 2012

Adgang til dokumentet

10.1094/asbcj-2012-1012-01

Citationsformater

@article{db5afbddd55a4d8d9db93d6c78341e74,

title = "Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation",

abstract = "A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.",

author = "Victor Abrahamsson and Signe Hoff and Nielsen, {Nikoline Juul} and Lametsch, {Marianne Lund} and Andersen, {Mogens Larsen}",

year = "2012",

doi = "10.1094/asbcj-2012-1012-01",

language = "English",

volume = "70",

pages = "296--302",

journal = "Journal of the American Society of Brewing Chemists",

issn = "0361-0470",

publisher = "Taylor & Francis",

number = "4",

}

TY - JOUR

T1 - Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

AU - Abrahamsson, Victor

AU - Hoff, Signe

AU - Nielsen, Nikoline Juul

AU - Lametsch, Marianne Lund

AU - Andersen, Mogens Larsen

PY - 2012

Y1 - 2012

N2 - A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

AB - A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

U2 - 10.1094/asbcj-2012-1012-01

DO - 10.1094/asbcj-2012-1012-01

M3 - Journal article

SN - 0361-0470

VL - 70

SP - 296

EP - 302

JO - Journal of the American Society of Brewing Chemists

JF - Journal of the American Society of Brewing Chemists

IS - 4

ER -

Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater