Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

Victor Abrahamsson; Signe Hoff; Nikoline Juul Nielsen; Marianne Lund Lametsch; Mogens Larsen Andersen

doi:10.1094/asbcj-2012-1012-01

Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

Victor Abrahamsson, Signe Hoff, Nikoline Juul Nielsen, Marianne Lund Lametsch, Mogens Larsen Andersen

19 Citations (Scopus)

Abstract

A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

Original language	English
Journal	Journal of the American Society of Brewing Chemists
Volume	70
Issue number	4
Pages (from-to)	296-302
Number of pages	7
ISSN	0361-0470
DOIs	https://doi.org/10.1094/asbcj-2012-1012-01
Publication status	Published - 2012

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title = "Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation",

abstract = "A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.",

author = "Victor Abrahamsson and Signe Hoff and Nielsen, {Nikoline Juul} and Lametsch, {Marianne Lund} and Andersen, {Mogens Larsen}",

year = "2012",

doi = "10.1094/asbcj-2012-1012-01",

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pages = "296--302",

journal = "Journal of the American Society of Brewing Chemists",

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T1 - Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

AU - Abrahamsson, Victor

AU - Hoff, Signe

AU - Nielsen, Nikoline Juul

AU - Lametsch, Marianne Lund

AU - Andersen, Mogens Larsen

PY - 2012

Y1 - 2012

N2 - A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

AB - A method was developed for quantification of sulfite in beer based on derivatization with the maleimide-derived probe ThioGlo 1 followed by separation of fluorescent adducts by reversed-phase high-performance liquid chromatography and fluorescence detection. Sulfite gave two ThioGlo 1 derivatives and it was shown by mass spectrometry that both had identical mass spectra. Matrix effects were observed when constructing sulfite standard curves in different beers and, therefore, use of a matrixmatched calibration curve is proposed. ThioGlo 1 was found to generate fluorescent adducts with both bound and free sulfite, providing a quantification of the total sulfite content in beer. The limit of quantification of sulfite was 0.6 mg/L and the method can be used for quantification of sulfite in highly colored beers.

U2 - 10.1094/asbcj-2012-1012-01

DO - 10.1094/asbcj-2012-1012-01

M3 - Journal article

SN - 0361-0470

VL - 70

SP - 296

EP - 302

JO - Journal of the American Society of Brewing Chemists

JF - Journal of the American Society of Brewing Chemists

IS - 4

ER -

Determination of sulfite in beer based on fluorescent derivatives and liquid chromatographic separation

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