Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Stefano Stella; Pablo Mesa; Johannes Thomsen; Bijoya Paul; Pablo Alcón; Simon Bo Jensen; Bhargav Saligram; Matias Emil Moses; Nikos S. Hatzakis; Guillermo Montoya

doi:10.1016/j.cell.2018.10.045

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Stefano Stella, Pablo Mesa, Johannes Thomsen, Bijoya Paul, Pablo Alcón, Simon Bo Jensen, Bhargav Saligram, Matias Emil Moses, Nikos S. Hatzakis, Guillermo Montoya

46 Citationer (Scopus)

Abstract

The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

Originalsprog	Engelsk
Tidsskrift	Cell
Vol/bind	175
Udgave nummer	7
Sider (fra-til)	1856-1871.e21
Antal sider	16
ISSN	0092-8674
DOI	https://doi.org/10.1016/j.cell.2018.10.045
Status	Udgivet - 13 dec. 2018

Adgang til dokumentet

10.1016/j.cell.2018.10.045

http://www.cell.com/article/S0092867418314016/pdf

Citationsformater

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abstract = "The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.",

author = "Stefano Stella and Pablo Mesa and Johannes Thomsen and Bijoya Paul and Pablo Alc{\'o}n and Jensen, {Simon Bo} and Bhargav Saligram and Moses, {Matias Emil} and Hatzakis, {Nikos S.} and Guillermo Montoya",

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T1 - Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

AU - Stella, Stefano

AU - Mesa, Pablo

AU - Thomsen, Johannes

AU - Paul, Bijoya

AU - Alcón, Pablo

AU - Jensen, Simon Bo

AU - Saligram, Bhargav

AU - Moses, Matias Emil

AU - Hatzakis, Nikos S.

AU - Montoya, Guillermo

PY - 2018/12/13

Y1 - 2018/12/13

N2 - The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

AB - The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

U2 - 10.1016/j.cell.2018.10.045

DO - 10.1016/j.cell.2018.10.045

M3 - Journal article

C2 - 30503205

SN - 0092-8674

VL - 175

SP - 1856-1871.e21

JO - Cell

JF - Cell

IS - 7

ER -

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Abstract

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