Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Stefano Stella; Pablo Mesa; Johannes Thomsen; Bijoya Paul; Pablo Alcón; Simon Bo Jensen; Bhargav Saligram; Matias Emil Moses; Nikos S. Hatzakis; Guillermo Montoya

doi:10.1016/j.cell.2018.10.045

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Stefano Stella, Pablo Mesa, Johannes Thomsen, Bijoya Paul, Pablo Alcón, Simon Bo Jensen, Bhargav Saligram, Matias Emil Moses, Nikos S. Hatzakis, Guillermo Montoya

46 Citations (Scopus)

Abstract

The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

Original language	English
Journal	Cell
Volume	175
Issue number	7
Pages (from-to)	1856-1871.e21
Number of pages	16
ISSN	0092-8674
DOIs	https://doi.org/10.1016/j.cell.2018.10.045
Publication status	Published - 13 Dec 2018

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http://www.cell.com/article/S0092867418314016/pdf

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title = "Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity",

abstract = "The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.",

author = "Stefano Stella and Pablo Mesa and Johannes Thomsen and Bijoya Paul and Pablo Alc{\'o}n and Jensen, {Simon Bo} and Bhargav Saligram and Moses, {Matias Emil} and Hatzakis, {Nikos S.} and Guillermo Montoya",

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T1 - Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

AU - Stella, Stefano

AU - Mesa, Pablo

AU - Thomsen, Johannes

AU - Paul, Bijoya

AU - Alcón, Pablo

AU - Jensen, Simon Bo

AU - Saligram, Bhargav

AU - Moses, Matias Emil

AU - Hatzakis, Nikos S.

AU - Montoya, Guillermo

PY - 2018/12/13

Y1 - 2018/12/13

N2 - The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

AB - The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.

U2 - 10.1016/j.cell.2018.10.045

DO - 10.1016/j.cell.2018.10.045

M3 - Journal article

C2 - 30503205

SN - 0092-8674

VL - 175

SP - 1856-1871.e21

JO - Cell

JF - Cell

IS - 7

ER -

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

Abstract

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