TY - JOUR
T1 - Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis
AU - Pedersen, Lotte Bang
AU - Ragkousi, K
AU - Cammett, T J
AU - Melly, E
AU - Sekowska, A
AU - Schopick, E
AU - Murray, T
AU - Setlow, P
N1 - Keywords: Amino Acid Sequence; Bacillus subtilis; Bacterial Proteins; Base Sequence; Carrier Proteins; Cell Division; Escherichia coli; Gene Expression Regulation, Bacterial; Hexosyltransferases; Lac Operon; Molecular Sequence Data; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutagenesis, Insertional; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Promoter Regions (Genetics); Recombinant Fusion Proteins; Sigma Factor; Spores, Bacterial
PY - 2000
Y1 - 2000
N2 - The Bacillus subtilis genome sequencing project [Kunst et al., Nature 390 (1997) 249-256] identified ywhE as a gene that potentially encodes a high-molecular-weight class A penicillin-binding protein. Analysis of the expression of a translational ywhE-lacZ fusion showed that ywhE expression is sporulation-specific, and is controlled predominantly by the forespore-specific sigma factor sigma(F), and to a lesser extent by sigma(G). Primer extension analysis identified two transcription start sites located 26 and 27 nucleotides upstream of the ywhE translational initiation codon. Sequences located in the -10 and -35 regions relative to the transcription start sites showed good homology to the consensus sequences for promoter elements of sigma(F)-dependent genes. An insertional mutation in ywhE had no significant effect on growth, morphology, and sporulation, and ywhE spores had normal heat-resistance, cortex structure, and germination and outgrowth properties. However, overexpression of ywhE in Escherichia coli resulted in cell lysis.
AB - The Bacillus subtilis genome sequencing project [Kunst et al., Nature 390 (1997) 249-256] identified ywhE as a gene that potentially encodes a high-molecular-weight class A penicillin-binding protein. Analysis of the expression of a translational ywhE-lacZ fusion showed that ywhE expression is sporulation-specific, and is controlled predominantly by the forespore-specific sigma factor sigma(F), and to a lesser extent by sigma(G). Primer extension analysis identified two transcription start sites located 26 and 27 nucleotides upstream of the ywhE translational initiation codon. Sequences located in the -10 and -35 regions relative to the transcription start sites showed good homology to the consensus sequences for promoter elements of sigma(F)-dependent genes. An insertional mutation in ywhE had no significant effect on growth, morphology, and sporulation, and ywhE spores had normal heat-resistance, cortex structure, and germination and outgrowth properties. However, overexpression of ywhE in Escherichia coli resulted in cell lysis.
U2 - 10.1016/S0378-1119(00)00084-6
DO - 10.1016/S0378-1119(00)00084-6
M3 - Journal article
C2 - 10767540
SN - 0378-1119
VL - 246
SP - 187
EP - 196
JO - Gene
JF - Gene
IS - 1-2
ER -