Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis

TY - JOUR

T1 - Characterization of ywhE, which encodes a putative high-molecular-weight class A penicillin-binding protein in Bacillus subtilis

AU - Pedersen, Lotte Bang

AU - Ragkousi, K

AU - Cammett, T J

AU - Melly, E

AU - Sekowska, A

AU - Schopick, E

AU - Murray, T

AU - Setlow, P

N1 - Keywords: Amino Acid Sequence; Bacillus subtilis; Bacterial Proteins; Base Sequence; Carrier Proteins; Cell Division; Escherichia coli; Gene Expression Regulation, Bacterial; Hexosyltransferases; Lac Operon; Molecular Sequence Data; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutagenesis, Insertional; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Promoter Regions (Genetics); Recombinant Fusion Proteins; Sigma Factor; Spores, Bacterial

PY - 2000

Y1 - 2000

N2 - The Bacillus subtilis genome sequencing project [Kunst et al., Nature 390 (1997) 249-256] identified ywhE as a gene that potentially encodes a high-molecular-weight class A penicillin-binding protein. Analysis of the expression of a translational ywhE-lacZ fusion showed that ywhE expression is sporulation-specific, and is controlled predominantly by the forespore-specific sigma factor sigma(F), and to a lesser extent by sigma(G). Primer extension analysis identified two transcription start sites located 26 and 27 nucleotides upstream of the ywhE translational initiation codon. Sequences located in the -10 and -35 regions relative to the transcription start sites showed good homology to the consensus sequences for promoter elements of sigma(F)-dependent genes. An insertional mutation in ywhE had no significant effect on growth, morphology, and sporulation, and ywhE spores had normal heat-resistance, cortex structure, and germination and outgrowth properties. However, overexpression of ywhE in Escherichia coli resulted in cell lysis.

AB - The Bacillus subtilis genome sequencing project [Kunst et al., Nature 390 (1997) 249-256] identified ywhE as a gene that potentially encodes a high-molecular-weight class A penicillin-binding protein. Analysis of the expression of a translational ywhE-lacZ fusion showed that ywhE expression is sporulation-specific, and is controlled predominantly by the forespore-specific sigma factor sigma(F), and to a lesser extent by sigma(G). Primer extension analysis identified two transcription start sites located 26 and 27 nucleotides upstream of the ywhE translational initiation codon. Sequences located in the -10 and -35 regions relative to the transcription start sites showed good homology to the consensus sequences for promoter elements of sigma(F)-dependent genes. An insertional mutation in ywhE had no significant effect on growth, morphology, and sporulation, and ywhE spores had normal heat-resistance, cortex structure, and germination and outgrowth properties. However, overexpression of ywhE in Escherichia coli resulted in cell lysis.

U2 - 10.1016/S0378-1119(00)00084-6

DO - 10.1016/S0378-1119(00)00084-6

M3 - Journal article

C2 - 10767540

SN - 0378-1119

VL - 246

SP - 187

EP - 196

JO - Gene

JF - Gene

IS - 1-2

ER -