TY - JOUR
T1 - Analysis of intracellular pH in Escherichia coli O157:H7 to determine the effect of chlorine dioxide decontamination
AU - Smigic, Nada
AU - Rajkovic, Andreja
AU - Arneborg, Nils
AU - Siegumfeldt, Henrik
AU - Devlieghere, Frank
AU - Nielsen, Dennis Sandris
PY - 2012/6
Y1 - 2012/6
N2 - The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO 2) by determining intracellular pH (pH i). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH i, colony counts were determined during the treatment with ClO 2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH i. The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO 2 increased.
AB - The aim of this study was to investigate the response of Escherichia coli O157:H7 when exposed to different concentrations of sanitation agent chlorine dioxide (ClO 2) by determining intracellular pH (pH i). For this purpose, fluorescence ratio imaging microscopy was used together with pH-sensitive, ratiometric green fluorescent protein that was introduced in E. coli O157:H7 cells. Along with pH i, colony counts were determined during the treatment with ClO 2. Results revealed several post-treatment subpopulations with different physiological states, as judged by their pH i. The fraction of cells with no pH gradient increased, and the colony count decreased as the concentration of ClO 2 increased.
U2 - 10.1007/s12161-011-9295-0
DO - 10.1007/s12161-011-9295-0
M3 - Journal article
SN - 1936-9751
VL - 5
SP - 327
EP - 331
JO - Food Analytical Methods
JF - Food Analytical Methods
IS - 3
ER -