Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer

Bo Højen Justesen; Randi Westh Hansen; Helle Juel Martens; Lisa Theorin; Michael Broberg Palmgren; Karen Laurence Martinez; Thomas Günther-Pomorski; Anja Thoe Fuglsang

doi:10.1074/jbc.m112.446948

Active plasma membrane P-type H⁺-ATPase reconstituted into nanodiscs is a monomer

Bo Højen Justesen, Randi Westh Hansen, Helle Juel Martens, Lisa Theorin, Michael Broberg Palmgren, Karen Laurence Martinez, Thomas Günther-Pomorski, Anja Thoe Fuglsang

14 Citationer (Scopus)

Abstract

Background: The plasma membrane H⁺-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H⁺-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H⁺-ATPase is a monomer. Significance: The plasma membrane H⁺-ATPase functions like well characterized cation pumping P-type ATPases.

Originalsprog	Engelsk
Tidsskrift	Journal of Biological Chemistry
Vol/bind	288
Udgave nummer	37
Sider (fra-til)	26419-26429
Antal sider	11
ISSN	0021-9258
DOI	https://doi.org/10.1074/jbc.m112.446948
Status	Udgivet - 13 sep. 2013

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10.1074/jbc.m112.446948

http://www.jbc.org/content/288/37/26419.full.pdfLicens: CC BY

Citationsformater

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abstract = "Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.",

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doi = "10.1074/jbc.m112.446948",

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AU - Justesen, Bo Højen

AU - Hansen, Randi Westh

AU - Martens, Helle Juel

AU - Theorin, Lisa

AU - Palmgren, Michael Broberg

AU - Martinez, Karen Laurence

AU - Günther-Pomorski, Thomas

AU - Fuglsang, Anja Thoe

PY - 2013/9/13

Y1 - 2013/9/13

N2 - Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.

AB - Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.

U2 - 10.1074/jbc.m112.446948

DO - 10.1074/jbc.m112.446948

M3 - Journal article

C2 - 23836891

SN - 0021-9258

VL - 288

SP - 26419

EP - 26429

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 37

ER -

Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer

Abstract

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Active plasma membrane P-type H⁺-ATPase reconstituted into nanodiscs is a monomer