Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer

Bo Højen Justesen; Randi Westh Hansen; Helle Juel Martens; Lisa Theorin; Michael Broberg Palmgren; Karen Laurence Martinez; Thomas Günther-Pomorski; Anja Thoe Fuglsang

doi:10.1074/jbc.m112.446948

Active plasma membrane P-type H⁺-ATPase reconstituted into nanodiscs is a monomer

Bo Højen Justesen, Randi Westh Hansen, Helle Juel Martens, Lisa Theorin, Michael Broberg Palmgren, Karen Laurence Martinez, Thomas Günther-Pomorski, Anja Thoe Fuglsang

14 Citations (Scopus)

Abstract

Background: The plasma membrane H⁺-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H⁺-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H⁺-ATPase is a monomer. Significance: The plasma membrane H⁺-ATPase functions like well characterized cation pumping P-type ATPases.

Original language	English
Journal	Journal of Biological Chemistry
Volume	288
Issue number	37
Pages (from-to)	26419-26429
Number of pages	11
ISSN	0021-9258
DOIs	https://doi.org/10.1074/jbc.m112.446948
Publication status	Published - 13 Sept 2013

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http://www.jbc.org/content/288/37/26419.full.pdfLicence: CC BY

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title = "Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer",

abstract = "Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.",

author = "Justesen, {Bo H{\o}jen} and Hansen, {Randi Westh} and Martens, {Helle Juel} and Lisa Theorin and Palmgren, {Michael Broberg} and Martinez, {Karen Laurence} and Thomas G{\"u}nther-Pomorski and Fuglsang, {Anja Thoe}",

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doi = "10.1074/jbc.m112.446948",

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AU - Justesen, Bo Højen

AU - Hansen, Randi Westh

AU - Martens, Helle Juel

AU - Theorin, Lisa

AU - Palmgren, Michael Broberg

AU - Martinez, Karen Laurence

AU - Günther-Pomorski, Thomas

AU - Fuglsang, Anja Thoe

PY - 2013/9/13

Y1 - 2013/9/13

N2 - Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.

AB - Background: The plasma membrane H+-ATPase generates electrochemical gradients in plants and fungi. The minimal subunit organization required for activity is not known. Results: We developed a protocol for reconstitution of active H+-ATPase in nanodiscs. Conclusion: The minimal functional unit of the H+-ATPase is a monomer. Significance: The plasma membrane H+-ATPase functions like well characterized cation pumping P-type ATPases.

U2 - 10.1074/jbc.m112.446948

DO - 10.1074/jbc.m112.446948

M3 - Journal article

C2 - 23836891

SN - 0021-9258

VL - 288

SP - 26419

EP - 26429

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 37

ER -

Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer

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Active plasma membrane P-type H⁺-ATPase reconstituted into nanodiscs is a monomer