A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

Rosa Laura Lopez Marques; Lisbeth Rosager Poulsen; Michael Broberg Palmgren

doi:10.1371/journal.pone.0033042

A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

Rosa Laura Lopez Marques, Lisbeth Rosager Poulsen, Michael Broberg Palmgren

26 Citationer (Scopus)

1533 Downloads (Pure)

Abstract

Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.

Originalsprog	Engelsk
Tidsskrift	P L o S One
Vol/bind	7
Udgave nummer	4
Antal sider	10
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0033042
Status	Udgivet - 13 apr. 2012

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10.1371/journal.pone.0033042

A Putative Plant Aminophospholipid Flippase, the Arabidopsis P4 ATPase ALA1, Localizes to the Plasma Membrane following Association with a b-SubunitForlagets udgivne version, 1,03 MB

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abstract = "Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.",

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AU - Poulsen, Lisbeth Rosager

AU - Palmgren, Michael Broberg

PY - 2012/4/13

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AB - Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.

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A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

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