A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

Rosa Laura Lopez Marques; Lisbeth Rosager Poulsen; Michael Broberg Palmgren

doi:10.1371/journal.pone.0033042

A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

Rosa Laura Lopez Marques, Lisbeth Rosager Poulsen, Michael Broberg Palmgren

26 Citations (Scopus)

1533 Downloads (Pure)

Abstract

Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.

Original language	English
Journal	P L o S One
Volume	7
Issue number	4
Number of pages	10
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0033042
Publication status	Published - 13 Apr 2012

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A Putative Plant Aminophospholipid Flippase, the Arabidopsis P4 ATPase ALA1, Localizes to the Plasma Membrane following Association with a b-SubunitFinal published version, 1.03 MB

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title = "A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a {\ss}-subunit",

abstract = "Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.",

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T1 - A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

AU - Lopez Marques, Rosa Laura

AU - Poulsen, Lisbeth Rosager

AU - Palmgren, Michael Broberg

PY - 2012/4/13

Y1 - 2012/4/13

N2 - Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.

AB - Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner leaflet and sphingolipids in the outer leaflet. This unequal distribution of lipids between leaflets is, amongst several proposed functions, hypothesized to be a prerequisite for endocytosis. P4 ATPases, belonging to the P-type ATPase superfamily of pumps, are involved in establishing lipid asymmetry across plasma membranes, but P4 ATPases have not been identified in plant plasma membranes. Here we report that the plant P4 ATPase ALA1, which previously has been connected with cold tolerance of Arabidopsis thaliana, is targeted to the plasma membrane and does so following association in the endoplasmic reticulum with an ALIS protein β-subunit.

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DO - 10.1371/journal.pone.0033042

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JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 4

ER -

A putative plant aminophospholipid flippase, the arabidopsis P4 ATPase ALA1, localizes to the plasma membrane following association with a ß-subunit

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