TY - JOUR
T1 - A comparative study of fine polished stainless steel, TiN and TiN/Ag surfaces
T2 - adhesion and attachment strength of Listeria monocytogenes as well as anti-listerial effect
AU - Skovager, Anne
AU - Whitehead, Kathryn
AU - Wickens, David
AU - Verran, Joanna
AU - Ingmer, Hanne
AU - Arneborg, Nils
PY - 2013
Y1 - 2013
N2 - Magnetron sputtering was used to produce nanocomposite TiN and TiN/Ag coatings on stainless steel surfaces. The surface chemistry (EDX), physicochemical properties (contact angles), topography and roughness parameters (WLP and AFM) of the fine polished stainless steel (FPSS), TiN and TiN/8.6. at.% Ag surfaces were examined. Real-time initial adhesion of two Listeria monocytogenes strains (EGDe and 64) to the three surfaces was determined under flow conditions, and their attachment strength after adhesion was measured using atomic force microscopy (AFM). The anti-listerial properties of the surfaces were determined using LIVE/DEAD staining.Our results demonstrate that FPSS, TiN and TiN/8.6. at.% Ag possessed different surface properties, which may influence both attachment strength and anti-listerial properties. There were no significant (p> 0.05) differences in the initial adhesion of the two L. monocytogenes strains to the three different surfaces. Attachment studies showed that the two L. monocytogenes strains did not attach to FPSS under wetted conditions. However, both strains attached to TiN and TiN/8.6. at.% Ag surfaces, although with less strength to TiN/8.6. at.% Ag than to TiN surfaces. The TiN/8.6. at.% Ag surface showed marked anti-listerial properties as compared with FPSS and TiN. Initial adhesion, attachment strength and anti-listerial properties were found to be strain dependent.
AB - Magnetron sputtering was used to produce nanocomposite TiN and TiN/Ag coatings on stainless steel surfaces. The surface chemistry (EDX), physicochemical properties (contact angles), topography and roughness parameters (WLP and AFM) of the fine polished stainless steel (FPSS), TiN and TiN/8.6. at.% Ag surfaces were examined. Real-time initial adhesion of two Listeria monocytogenes strains (EGDe and 64) to the three surfaces was determined under flow conditions, and their attachment strength after adhesion was measured using atomic force microscopy (AFM). The anti-listerial properties of the surfaces were determined using LIVE/DEAD staining.Our results demonstrate that FPSS, TiN and TiN/8.6. at.% Ag possessed different surface properties, which may influence both attachment strength and anti-listerial properties. There were no significant (p> 0.05) differences in the initial adhesion of the two L. monocytogenes strains to the three different surfaces. Attachment studies showed that the two L. monocytogenes strains did not attach to FPSS under wetted conditions. However, both strains attached to TiN and TiN/8.6. at.% Ag surfaces, although with less strength to TiN/8.6. at.% Ag than to TiN surfaces. The TiN/8.6. at.% Ag surface showed marked anti-listerial properties as compared with FPSS and TiN. Initial adhesion, attachment strength and anti-listerial properties were found to be strain dependent.
U2 - 10.1016/j.colsurfb.2013.03.044
DO - 10.1016/j.colsurfb.2013.03.044
M3 - Journal article
C2 - 23643915
SN - 0927-7765
VL - 109
SP - 190
EP - 196
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -