53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress

TY - JOUR

T1 - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress

AU - Lukas, Claudia

AU - Savic, Velibor

AU - Bekker-Jensen, Simon

AU - Doil, Carsten

AU - Neumann, Beate

AU - Pedersen, Ronni Sølvhøi

AU - Grøfte, Merete

AU - Chan, Kok Lung

AU - Hickson, Ian David

AU - Bartek, Jiri

AU - Lukas, Jiri

N1 - © 2011 Macmillan Publishers Limited. All rights reserved.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Completion of genome duplication is challenged by structural and topological barriers that impede progression of replication forks. Although this can seriously undermine genome integrity, the fate of DNA with unresolved replication intermediates is not known. Here, we show that mild replication stress increases the frequency of chromosomal lesions that are transmitted to daughter cells. Throughout G1, these lesions are sequestered in nuclear compartments marked by p53-binding protein 1 (53BP1) and other chromatin-associated genome caretakers. We show that the number of such 53BP1 nuclear bodies increases after genetic ablation of BLM, a DNA helicase associated with dissolution of entangled DNA. Conversely, 53BP1 nuclear bodies are partially suppressed by knocking down SMC2, a condensin subunit required for mechanical stability of mitotic chromosomes. Finally, we provide evidence that 53BP1 nuclear bodies shield chromosomal fragile sites sequestered in these compartments against erosion. Together, these data indicate that restoration of DNA or chromatin integrity at loci prone to replication problems requires mitotic transmission to the next cell generations.

AB - Completion of genome duplication is challenged by structural and topological barriers that impede progression of replication forks. Although this can seriously undermine genome integrity, the fate of DNA with unresolved replication intermediates is not known. Here, we show that mild replication stress increases the frequency of chromosomal lesions that are transmitted to daughter cells. Throughout G1, these lesions are sequestered in nuclear compartments marked by p53-binding protein 1 (53BP1) and other chromatin-associated genome caretakers. We show that the number of such 53BP1 nuclear bodies increases after genetic ablation of BLM, a DNA helicase associated with dissolution of entangled DNA. Conversely, 53BP1 nuclear bodies are partially suppressed by knocking down SMC2, a condensin subunit required for mechanical stability of mitotic chromosomes. Finally, we provide evidence that 53BP1 nuclear bodies shield chromosomal fragile sites sequestered in these compartments against erosion. Together, these data indicate that restoration of DNA or chromatin integrity at loci prone to replication problems requires mitotic transmission to the next cell generations.

U2 - 10.1038/ncb2201

DO - 10.1038/ncb2201

M3 - Journal article

C2 - 21317883

SN - 1465-7392

VL - 13

SP - 243

EP - 253

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 3

ER -