Bibliografisk note

Funding Information:
This work was supported by ERC Consolidator Award 681396 Extinction Genomics, DNRF143 Center for Evolutionary Hologenomics, and the Norwegian Environment Agency (project 18088069 ). The National Science Centre, Poland supported S. Nowak (grant No. 2020/39/B/NZ9/01829 ) and R.W. Mysłajek (grant No. 2019/35/O/NZ8/01550 ). G.H-A. is supported by the Consejo Nacional de Ciencia y Tecnología from Mexico ( CONACyT 576734 ). R.Godinho is supported by the Portuguese Foundation for science and Technology ( DL57/2016/CP1440 ). U. Saarma and M. Hindrikson were supported by funding from the Estonian Ministry of Education and Research (grants PRG1209 and PSG715 ). We would like to thank Luca Sineo for helping collect the Sicilian wolf samples and Domenico Tricomi, together with the owners of the Cirneco dell’Etna dogs for their collaboration in this project. We are deeply grateful to the curators of the museums who allowed us to sample the historical Sicilian wolves: Paolo Agnelli of the Museum of Natural History of the University of Florence, zoology section "La Specola", Fabio Lo Bono of the Civic Museum "Baldassare Romano" in Termini Imerese (PA), Enrico Bellia of the Museum of Zoology "P. Doderlein", SIMUA, Palermo (PA) and Ferdinando Maurici and Fabio Lo Valvo from Regional Museum of Terrasini (PA). We also acknowledge Davide Palumbo for his valuable inputs and support at the beginning of this collaboration. We also thank the staff of the Danish National High-throughput Sequencing Center and BGI Denmark for their support in data generation and the Danish National Supercomputer for Life Sciences at the DTU National Lifescience Center at Technical University of Denmark (DTU) for facilitating the data analysis process.

Funding Information:
This work was supported by ERC Consolidator Award 681396 Extinction Genomics, DNRF143 Center for Evolutionary Hologenomics, and the Norwegian Environment Agency (project 18088069). The National Science Centre, Poland supported S. Nowak (grant No. 2020/39/B/NZ9/01829) and R.W. Mysłajek (grant No. 2019/35/O/NZ8/01550). G.H-A. is supported by the Consejo Nacional de Ciencia y Tecnología from Mexico (CONACyT 576734). R.Godinho is supported by the Portuguese Foundation for science and Technology (DL57/2016/CP1440). U. Saarma and M. Hindrikson were supported by funding from the Estonian Ministry of Education and Research (grants PRG1209 and PSG715). We would like to thank Luca Sineo for helping collect the Sicilian wolf samples and Domenico Tricomi, together with the owners of the Cirneco dell'Etna dogs for their collaboration in this project. We are deeply grateful to the curators of the museums who allowed us to sample the historical Sicilian wolves: Paolo Agnelli of the Museum of Natural History of the University of Florence, zoology section "La Specola", Fabio Lo Bono of the Civic Museum “Baldassare Romano” in Termini Imerese (PA), Enrico Bellia of the Museum of Zoology "P. Doderlein", SIMUA, Palermo (PA) and Ferdinando Maurici and Fabio Lo Valvo from Regional Museum of Terrasini (PA). We also acknowledge Davide Palumbo for his valuable inputs and support at the beginning of this collaboration. We also thank the staff of the Danish National High-throughput Sequencing Center and BGI Denmark for their support in data generation and the Danish National Supercomputer for Life Sciences at the DTU National Lifescience Center at Technical University of Denmark (DTU) for facilitating the data analysis process. This genome scale study of the Sicilian wolf was initiated by M.M.C. E.C. and M.T.P.G. to build upon the findings of a prior mtDNA analyses of the Sicilian wolf led by F.M.A. and L.R. M.M.C. J.R.-M. M.H-S.S. E.C, and S.G. designed the research. F.M.A. L.R. E.C. S.L.B. and M.M.C. contributed with material, information, and logistics to the historical Sicilian samples. J.A. I.K. La.B. Li.B. J.M. M.A. U.S. M.H. P.H. B.C.B. C.N. R.Q. S.S. L.P. S.N. R.W.M. S.L.B. P.C. Lu.B. C.V. H.K.S. and O.S. contributed material information, logistic and/or contributed to modern samples. M.M.C. did historical DNA molecular work, supervised by O.S. M.H.S.S. and M.T.P.G. C.H.S.-O. L.T.L. I.F. and C.G.C. coordinated logistics and/or did modern DNA molecular work, supervised by M.M.C. M.H.S.S. and M.T.P.G. M.M.C. and G.H.-A. did initial processing of the modern raw sequencing data, supervised by S.G. and M.T.P.G. M.M.C. performed the population genomic, nucleotide diversity, and heterozygosity analyses with input from J.R.-M. A.C. and J.R.-M did admixture graph analyses. S.G.A. performed the runs of homozygosity and genomic load analyses, with input from L.F. X.S. performed the local ancestry inference analysis. M.M.C. wrote the paper with input from J.R.-M. S.G. L.R. F.M.A, M.-H.S.S. and all other authors. The authors declare no competing interests.

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