ETV2 induces endothelial, but not hematopoietic, lineage specification in birds
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ETV2 induces endothelial, but not hematopoietic, lineage specification in birds. / Weng, Wei; Deng, Yuan; Deviatiiarov, Ruslan; Hamidi, Sofiane; Kajikawa, Eriko; Gusev, Oleg; Kiyonari, Hiroshi; Zhang, Guojie; Sheng, Guojun.
I: Life Science Alliance, Bind 7, Nr. 6, e202402694, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - ETV2 induces endothelial, but not hematopoietic, lineage specification in birds
AU - Weng, Wei
AU - Deng, Yuan
AU - Deviatiiarov, Ruslan
AU - Hamidi, Sofiane
AU - Kajikawa, Eriko
AU - Gusev, Oleg
AU - Kiyonari, Hiroshi
AU - Zhang, Guojie
AU - Sheng, Guojun
N1 - Publisher Copyright: © 2024 Weng et al.
PY - 2024
Y1 - 2024
N2 - Cardiovascular system develops from the lateral plate mesoderm. Its three primary cell lineages (hematopoietic, endothelial, and muscular) are specified by the sequential actions of conserved transcriptional factors. ETV2, a master regulator of mammalian hemangioblast development, however, is absent in the chicken genome and acts downstream of NPAS4L in zebrafish. Here, we investigated the epistatic relationship between NPAS4L and ETV2 in avian hemangioblast development. We showed that ETV2 is deleted in all 363 avian genomes analyzed. Mouse ETV2 induced LMO2, but not NPAS4L or SCL, expression in chicken mesoderm. Squamate (lizards, geckos, and snakes) genomes contain both NPAS4L and ETV2 In Madagascar ground gecko, both genes were expressed in developing hemangioblasts. Gecko ETV2 induced only LMO2 in chicken mesoderm. We propose that both NPAS4L and ETV2 were present in ancestral amniote, with ETV2 acting downstream of NPAS4L in endothelial lineage specification. ETV2 may have acted as a pioneer factor by promoting chromatin accessibility of endothelial-specific genes and, in parallel with NPAS4L loss in ancestral mammals, has gained similar function in regulating blood-specific genes.
AB - Cardiovascular system develops from the lateral plate mesoderm. Its three primary cell lineages (hematopoietic, endothelial, and muscular) are specified by the sequential actions of conserved transcriptional factors. ETV2, a master regulator of mammalian hemangioblast development, however, is absent in the chicken genome and acts downstream of NPAS4L in zebrafish. Here, we investigated the epistatic relationship between NPAS4L and ETV2 in avian hemangioblast development. We showed that ETV2 is deleted in all 363 avian genomes analyzed. Mouse ETV2 induced LMO2, but not NPAS4L or SCL, expression in chicken mesoderm. Squamate (lizards, geckos, and snakes) genomes contain both NPAS4L and ETV2 In Madagascar ground gecko, both genes were expressed in developing hemangioblasts. Gecko ETV2 induced only LMO2 in chicken mesoderm. We propose that both NPAS4L and ETV2 were present in ancestral amniote, with ETV2 acting downstream of NPAS4L in endothelial lineage specification. ETV2 may have acted as a pioneer factor by promoting chromatin accessibility of endothelial-specific genes and, in parallel with NPAS4L loss in ancestral mammals, has gained similar function in regulating blood-specific genes.
U2 - 10.26508/lsa.202402694
DO - 10.26508/lsa.202402694
M3 - Journal article
C2 - 38570190
AN - SCOPUS:85190076290
VL - 7
JO - Life Science Alliance
JF - Life Science Alliance
SN - 2575-1077
IS - 6
M1 - e202402694
ER -
ID: 389422030