Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
CRISPR-mediated endogenous tagging of genes provides unique possibilities to explore the function and dynamic subcellular localization of proteins in living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.
Originalsprog | Engelsk |
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Titel | Methods in Molecular Biology |
Antal sider | 20 |
Forlag | Humana Press |
Publikationsdato | 2024 |
Sider | 147-166 |
Kapitel | 9 |
ISBN (Trykt) | 978-1-0716-3506-3 |
ISBN (Elektronisk) | 978-1-0716-3507-0 |
DOI | |
Status | Udgivet - 2024 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2725 |
ISSN | 1064-3745 |
Bibliografisk note
Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
ID: 373795328