Bacterial bioindicators enable biological status classification along the continental Danube river

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  • Laurent Fontaine
  • Lorenzo Pin
  • Domenico Savio
  • Nikolai Friberg
  • Alexander K.T. Kirschner
  • Andreas H. Farnleitner
  • Alexander Eiler
Despite the importance of bacteria in aquatic ecosystems and their predictable diversity patterns across space and time, biomonitoring tools for status assessment relying on these organisms are widely lacking. This is partly due to insufficient data and models to identify reliable microbial predictors. Here, we show metabarcoding in combination with multivariate statistics and machine learning allows to identify bacterial bioindicators for existing biological status classification systems. Bacterial beta-diversity dynamics follow environmental gradients and the observed associations highlight potential bioindicators for ecological outcomes. Spatio-temporal links spanning the microbial communities along the river allow accurate prediction of downstream biological status from upstream information. Network analysis on amplicon sequence veariants identify as good indicators genera Fluviicola, Acinetobacter, Flavobacterium, and Rhodoluna, and reveal informational redundancy among taxa, which coincides with taxonomic relatedness. The redundancy among bacterial bioindicators reveals mutually exclusive taxa, which allow accurate biological status modeling using as few as 2-3 amplicon sequence variants. As such our models show that using a few bacterial amplicon sequence variants from globally distributed genera allows for biological status assessment along river systems.
OriginalsprogEngelsk
Artikelnummer862
TidsskriftCommunications Biology
Vol/bind6
Udgave nummer1
Antal sider11
ISSN2399-3642
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This study has been supported financially by the Department of Biosciences and the Centre of Biogeochemistry in the Anthropocene, University of Oslo (“Startpakke” of Alexander Eiler). This research is part of the EuroFLOW project (EUROpean training and research network for environmental FLOW management in river basins) funded by the European Union’s Horizon 2020 - Research and Innovation Framework Programme under the Marie Skłodowska-Curie grant agreement (MSCA) No.765553. This study was supported by the Austrian Science Fund (FWF) as part of the “Vienna Doctoral Program on Water Resource Systems” (DKplus W1219-N22) and the FWF projects P25817-B22, P23900-B22 and P32464-B, as well as the research project “Groundwater Resource Systems Vienna” in cooperation with Vienna Water (MA31). Infrastructure (cruise ships, floating laboratory) and logistics for collecting, storing and transporting samples were provided by the International Commission for the Protection of the Danube River (ICPDR). This manuscript includes data licensed by the ICPDR, retrieved from the Danubis database (https://danubis.icpdr.org/). We specifically thank Wolfram Graf, Béla Csány, Patrick Leitner, Momir Paunovic, Thomas Huber, Joszef Szekeres, Claudia Nagy and Peter Borza for providing macroinvertebrate data and saprobic index classification, Martin Dokulil and Ulrich Donabaum for providing chlorophyll a data, Carmen Hamchivici, Florentina Dumitrache, Fabio Sena, Günther Umlauf, Carmen Postolache and Ion Udrea for providing general physico-chemical parameters and nutrients as well as Marija Marjanovic-Rajcic and Damir Thomas for providing DOC data. We thank Franck Lejzerowicz for insightful suggestions which improved the manuscript.

Funding Information:
This study has been supported financially by the Department of Biosciences and the Centre of Biogeochemistry in the Anthropocene, University of Oslo (“Startpakke” of Alexander Eiler). This research is part of the EuroFLOW project (EUROpean training and research network for environmental FLOW management in river basins) funded by the European Union’s Horizon 2020 - Research and Innovation Framework Programme under the Marie Skłodowska-Curie grant agreement (MSCA) No.765553. This study was supported by the Austrian Science Fund (FWF) as part of the “Vienna Doctoral Program on Water Resource Systems” (DKplus W1219-N22) and the FWF projects P25817-B22, P23900-B22 and P32464-B, as well as the research project “Groundwater Resource Systems Vienna” in cooperation with Vienna Water (MA31). Infrastructure (cruise ships, floating laboratory) and logistics for collecting, storing and transporting samples were provided by the International Commission for the Protection of the Danube River (ICPDR). This manuscript includes data licensed by the ICPDR, retrieved from the Danubis database ( https://danubis.icpdr.org/ ). We specifically thank Wolfram Graf, Béla Csány, Patrick Leitner, Momir Paunovic, Thomas Huber, Joszef Szekeres, Claudia Nagy and Peter Borza for providing macroinvertebrate data and saprobic index classification, Martin Dokulil and Ulrich Donabaum for providing chlorophyll a data, Carmen Hamchivici, Florentina Dumitrache, Fabio Sena, Günther Umlauf, Carmen Postolache and Ion Udrea for providing general physico-chemical parameters and nutrients as well as Marija Marjanovic-Rajcic and Damir Thomas for providing DOC data. We thank Franck Lejzerowicz for insightful suggestions which improved the manuscript.

Publisher Copyright:
© 2023, Springer Nature Limited.

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