Working with RNA

TY - CHAP

T1 - Working with RNA

AU - Nielsen, Henrik

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Working with RNA is not a special discipline in molecular biology. However, RNA is chemically and structurally different from DNA and a few simple work rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modifications to standard protocols in molecular biology that are relevant to RNA work. The methods are applicable to all types of RNA and require a minimum of specialized equipment.

AB - Working with RNA is not a special discipline in molecular biology. However, RNA is chemically and structurally different from DNA and a few simple work rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modifications to standard protocols in molecular biology that are relevant to RNA work. The methods are applicable to all types of RNA and require a minimum of specialized equipment.

KW - Chemistry Techniques, Analytical

KW - Diethyl Pyrocarbonate

KW - Electrophoresis

KW - Genetic Techniques

KW - Hydrogen-Ion Concentration

KW - Metals

KW - Nucleotides

KW - Preservation, Biological

KW - RNA

KW - Ribonucleases

KW - Spectrophotometry, Ultraviolet

KW - Temperature

U2 - 10.1007/978-1-59745-248-9_2

DO - 10.1007/978-1-59745-248-9_2

M3 - Book chapter

C2 - 21125480

VL - 703

SP - 15

EP - 28

BT - Methods in Molecular Biology

PB - Springer Science+Business Media

ER -