TY - JOUR
T1 - Visualization by mass spectrometry of 2-dimensional changes in rat brain lipids, including N-acylphosphatidylethanolamines, during neonatal brain ischemia
AU - Janfelt, Christian
AU - Wellner, Niels
AU - Leger, Pierre-Louis
AU - Kokesch-Himmelreich, Julia
AU - Hansen, Steen Honore'
AU - Charriaut-Marlangue, Christiane
AU - Hansen, Harald S.
PY - 2012/6
Y1 - 2012/6
N2 - Spatial synthesis of N-acyl-phosphatidylethanolamines (NAPEs) and N-acylethanolamines (NAEs) during ischemia-reperfusion in neonatal rats has been investigated and compared to the spatial degradation of other phospholipids. Ischemia was induced in anesthetized Wistar P7 rat pups by left middle cerebral artery electrocoagulation combined with a transient and concomitant occlusion of both common carotid arteries. Pups were sacrificed after 24 and 48 h. Sham-treated animals were sacrificed after 48 h. The frozen brains were sliced and subjected to desorption electrospray ionization imaging mass spectrometry. There was a remarkable increase in the levels of many species of NAPEs in the whole injured area at both time points, and a clear but minor increase in selected NAEs. In the ischemic area, the sodium adducts of phosphatidylcholine and of lyso-phosphatidylcholine accumulated and the potassium adduct of phosphatidylcholine disappeared, indicating breakdown of the Na +/K+pump. Free fatty acids, e.g., arachidonic and docosahexaenoic acids, tended to be more abundant in the periphery than in the center of the ischemic area and showed different spatial distribution. NAPEs are synthesized in the whole ischemic area where the cells seem to be dead and other phospholipids are degraded. Free fatty acids can be found in the periphery of the ischemic area.
AB - Spatial synthesis of N-acyl-phosphatidylethanolamines (NAPEs) and N-acylethanolamines (NAEs) during ischemia-reperfusion in neonatal rats has been investigated and compared to the spatial degradation of other phospholipids. Ischemia was induced in anesthetized Wistar P7 rat pups by left middle cerebral artery electrocoagulation combined with a transient and concomitant occlusion of both common carotid arteries. Pups were sacrificed after 24 and 48 h. Sham-treated animals were sacrificed after 48 h. The frozen brains were sliced and subjected to desorption electrospray ionization imaging mass spectrometry. There was a remarkable increase in the levels of many species of NAPEs in the whole injured area at both time points, and a clear but minor increase in selected NAEs. In the ischemic area, the sodium adducts of phosphatidylcholine and of lyso-phosphatidylcholine accumulated and the potassium adduct of phosphatidylcholine disappeared, indicating breakdown of the Na +/K+pump. Free fatty acids, e.g., arachidonic and docosahexaenoic acids, tended to be more abundant in the periphery than in the center of the ischemic area and showed different spatial distribution. NAPEs are synthesized in the whole ischemic area where the cells seem to be dead and other phospholipids are degraded. Free fatty acids can be found in the periphery of the ischemic area.
M3 - Journal article
SN - 0892-6638
VL - 26
SP - 2667
EP - 2673
JO - F A S E B Journal
JF - F A S E B Journal
IS - 6
ER -