Virus-activated T cells regulate expression of adhesion molecules on endothelial cells in sites of infection

TY - JOUR

T1 - Virus-activated T cells regulate expression of adhesion molecules on endothelial cells in sites of infection

AU - Marker, O

AU - Scheynius, A

AU - Christensen, Jan Pravsgaard

AU - Thomsen, Allan Randrup

N1 - Keywords: Animals; CD8-Positive T-Lymphocytes; Choroid Plexus; Endothelium; Female; Gene Expression Regulation, Viral; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II; Immunohistochemistry; Intercellular Adhesion Molecule-1; Lymphocytic choriomeningitis virus; Meninges; Meningitis; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Nude; Vascular Cell Adhesion Molecule-1

PY - 1995

Y1 - 1995

N2 - To study the role of cell adhesion molecules in the fatal CD8+ T-cell mediated meningitis which is induced by intracerebral infection with lymphocytic choriomeningitis virus, the expression of relevant molecules on inflammatory cells and local endothelium was analyzed immunohistochemically. Most inflammatory cells were strongly positive for LFA-1, VLA-4, Pgp-1 and ICAM-1. Expression of ICAM-1 and VCAM-1 was upregulated on the endothelial cells in immunocompetent mice, but not in T-cell deficient nude mice. Analysis of mice deficient in either CD4+ or CD8+ T cells, revealed that not only was the inflammatory reaction dependent on the presence of CD8+ cells, but these cells also appeared to be required for maximal upregulation of ICAM-1 and VCAM-1 on the endothelial cells. These results indicate that virus-specific CD8+ T cells are crucially involved in regulating the inflammatory reaction through effects on endothelial expression of adhesion molecules.

AB - To study the role of cell adhesion molecules in the fatal CD8+ T-cell mediated meningitis which is induced by intracerebral infection with lymphocytic choriomeningitis virus, the expression of relevant molecules on inflammatory cells and local endothelium was analyzed immunohistochemically. Most inflammatory cells were strongly positive for LFA-1, VLA-4, Pgp-1 and ICAM-1. Expression of ICAM-1 and VCAM-1 was upregulated on the endothelial cells in immunocompetent mice, but not in T-cell deficient nude mice. Analysis of mice deficient in either CD4+ or CD8+ T cells, revealed that not only was the inflammatory reaction dependent on the presence of CD8+ cells, but these cells also appeared to be required for maximal upregulation of ICAM-1 and VCAM-1 on the endothelial cells. These results indicate that virus-specific CD8+ T cells are crucially involved in regulating the inflammatory reaction through effects on endothelial expression of adhesion molecules.

M3 - Journal article

C2 - 7499490

SN - 0165-5728

VL - 62

SP - 35

EP - 42

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1

ER -