VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

Mette Grønlund

doi:10.1007/978-1-4939-2453-0_19

VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

Mette Grønlund^*

^*Corresponding author for this work

Section for Plant and Soil Sciences

1 Citation (Scopus)

Abstract

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.

Original language	English
Title of host publication	Plant gene silencing : methods and protocols
Editors	Kirankumar S. Mysore, Muthappa Senthil-Kumar
Number of pages	11
Volume	Part II
Publisher	Springer
Publication date	2015
Pages	255-265
Chapter	19
ISBN (Print)	978-1-4939-2452-3
ISBN (Electronic)	978-1-4939-2453-0
DOIs	https://doi.org/10.1007/978-1-4939-2453-0_19
Publication status	Published - 2015

Series	Methods in Molecular Biology
Volume	1287
ISSN	1064-3745

Keywords

Arbuscular mycorrhizal symbiosis
Legume- Rhizobium symbiosis
PEBV
VIGS

Access to Document

10.1007/978-1-4939-2453-0_19

Cite this

VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants. / Grønlund, Mette.

Plant gene silencing: methods and protocols. ed. / Kirankumar S. Mysore; Muthappa Senthil-Kumar. Vol. Part II Springer, 2015. p. 255-265 (Methods in Molecular Biology, Vol. 1287).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

@inbook{e357aa1e59d54431a5f74ec4f0c183b5,

title = "VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants",

abstract = "Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.",

keywords = "Arbuscular mycorrhizal symbiosis, Legume- Rhizobium symbiosis, PEBV, VIGS",

author = "Mette Gr{\o}nlund",

year = "2015",

doi = "10.1007/978-1-4939-2453-0_19",

language = "English",

isbn = "978-1-4939-2452-3",

volume = "Part II",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "255--265",

editor = "Mysore, {Kirankumar S.} and Muthappa Senthil-Kumar",

booktitle = "Plant gene silencing",

}

TY - CHAP

T1 - VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

AU - Grønlund, Mette

PY - 2015

Y1 - 2015

N2 - Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.

AB - Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.

KW - Arbuscular mycorrhizal symbiosis

KW - Legume- Rhizobium symbiosis

KW - PEBV

KW - VIGS

U2 - 10.1007/978-1-4939-2453-0_19

DO - 10.1007/978-1-4939-2453-0_19

M3 - Book chapter

C2 - 25740371

AN - SCOPUS:84924362533

SN - 978-1-4939-2452-3

VL - Part II

T3 - Methods in Molecular Biology

SP - 255

EP - 265

BT - Plant gene silencing

A2 - Mysore, Kirankumar S.

A2 - Senthil-Kumar, Muthappa

PB - Springer

ER -

VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

Abstract

Keywords

Access to Document

Fingerprint

Cite this