VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

Mette Grønlund*

*Corresponding author af dette arbejde
1 Citationer (Scopus)

Abstract

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are diffi cult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.

OriginalsprogEngelsk
TitelPlant gene silencing : methods and protocols
RedaktørerKirankumar S. Mysore, Muthappa Senthil-Kumar
Antal sider11
Vol/bindPart II
ForlagSpringer
Publikationsdato2015
Sider255-265
Kapitel19
ISBN (Trykt)978-1-4939-2452-3
ISBN (Elektronisk)978-1-4939-2453-0
DOI
StatusUdgivet - 2015
NavnMethods in Molecular Biology
Vol/bind1287
ISSN1064-3745

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