Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments

Annette Bruun Jensen; J. Malagocka; J. Eilenberg; B.l. Fredensborg

doi:10.3920/JIFF2016.0042

Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments

Annette Bruun Jensen, J. Malagocka, J. Eilenberg, B.l. Fredensborg

Section for Organismal Biology

Abstract

In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematode Dicrocoelium dendriticum metacercariae from the abdomen of the red wood ant Formica polyctena. Then we tested the viability of D. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all control ants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver fluke D. dendriticum metacercariae.

Original language	English
Journal	Journal of Insects as Food and Feed
Volume	3
Issue number	1
Pages (from-to)	15-20
Number of pages	6
ISSN	2352-4588
DOIs	https://doi.org/10.3920/JIFF2016.0042
Publication status	Published - 2017

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@article{9c065135cd564223b66bb7019ac10d5e,

title = "Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments",

abstract = "In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematode Dicrocoelium dendriticum metacercariae from the abdomen of the red wood ant Formica polyctena. Then we tested the viability of D. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all control ants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver fluke D. dendriticum metacercariae.",

author = "Jensen, {Annette Bruun} and J. Malagocka and J. Eilenberg and B.l. Fredensborg",

year = "2017",

doi = "10.3920/JIFF2016.0042",

language = "English",

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pages = "15--20",

journal = "Journal of Insects as Food and Feed",

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publisher = "Wageningen Academic Publishers",

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T1 - Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments

AU - Jensen, Annette Bruun

AU - Malagocka, J.

AU - Eilenberg, J.

AU - Fredensborg, B.l.

PY - 2017

Y1 - 2017

N2 - In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematode Dicrocoelium dendriticum metacercariae from the abdomen of the red wood ant Formica polyctena. Then we tested the viability of D. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all control ants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver fluke D. dendriticum metacercariae.

AB - In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematode Dicrocoelium dendriticum metacercariae from the abdomen of the red wood ant Formica polyctena. Then we tested the viability of D. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all control ants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver fluke D. dendriticum metacercariae.

U2 - 10.3920/JIFF2016.0042

DO - 10.3920/JIFF2016.0042

M3 - Journal article

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VL - 3

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JO - Journal of Insects as Food and Feed

JF - Journal of Insects as Food and Feed

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ER -

Viability of Dicrocoelium dendriticum metacercariae in Formica polyctena ants after exposure to different treatments

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