UV Photodissociation Mass Spectrometry Accurately Localize Sites of Backbone Deuteration in Peptides

TY - JOUR

T1 - UV Photodissociation Mass Spectrometry Accurately Localize Sites of Backbone Deuteration in Peptides

AU - Mistarz, Ulrik Hvid

AU - Bellina, Bruno

AU - Jensen, Pernille Foged

AU - Brown, Jeffery M.

AU - Barran, Perdita E

AU - Rand, Kasper D

PY - 2018/1/16

Y1 - 2018/1/16

N2 - Hydrogen/deuterium exchange mass spectrometry (HDX-MS) is now a routinely used technique to inform on protein structure, dynamics, and interactions. Localizing the incorporated deuterium content on a single residue basis increases the spatial resolution of this technique enabling detailed structural analysis. Here, we investigate the use of ultraviolet photodissociation (UVPD) at 213 nm to measure deuterium levels at single residue resolution in HDX-MS experiments. Using a selectively labeled peptide, we show that UVPD occurs without H/D scrambling as the peptide probe accurately retains its solution-phase deuterium labeling pattern. Our results indicate that UVPD provides an attractive alternative to electron mediated dissociation for increasing the spatial resolution of the HDX-MS experiment, capable of yielding high fragmentation efficiency, high fragment ion diversity, and low precursor ion charge-state dependency.

AB - Hydrogen/deuterium exchange mass spectrometry (HDX-MS) is now a routinely used technique to inform on protein structure, dynamics, and interactions. Localizing the incorporated deuterium content on a single residue basis increases the spatial resolution of this technique enabling detailed structural analysis. Here, we investigate the use of ultraviolet photodissociation (UVPD) at 213 nm to measure deuterium levels at single residue resolution in HDX-MS experiments. Using a selectively labeled peptide, we show that UVPD occurs without H/D scrambling as the peptide probe accurately retains its solution-phase deuterium labeling pattern. Our results indicate that UVPD provides an attractive alternative to electron mediated dissociation for increasing the spatial resolution of the HDX-MS experiment, capable of yielding high fragmentation efficiency, high fragment ion diversity, and low precursor ion charge-state dependency.

KW - Journal Article

U2 - 10.1021/acs.analchem.7b04683

DO - 10.1021/acs.analchem.7b04683

M3 - Journal article

C2 - 29266933

SN - 0003-2700

VL - 90

SP - 1077

EP - 1080

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -