Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG

Pia Afzelius; Aage Ko Alstrup; Henrik C Schønheyder; Per Borghammer; Svend B Jensen; Dirk Bender; Ole L Nielsen

Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG

Pia Afzelius, Aage Ko Alstrup, Henrik C Schønheyder, Per Borghammer, Svend B Jensen, Dirk Bender, Ole L Nielsen

23 Downloads (Pure)

Abstract

The aim of this study was to compare (11)C-methionine and (11)C-donepezil positron emission tomography (PET) with (111)In-labeled leukocyte and (99m) Tc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and (18)F-fluorodeoxyglucose ((18)F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus. The sequential scan protocol included Computed Tomography, (11)C-methionine and (11)C-donepezil PET, (99m) Tc-DPD and (111)In-labelled leukocytes scintigraphy, and (18)F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions, (18)F-FDG accumulated in 100%, (111)In-leukocytes in 79%, (11)C-methionine in 79%, (11)C-donepezil in 58%, and (99m) Tc-DPD in none. Overall, (18)F-FDG PET was superior to (111)In-leukocyte SPECT and (11)C-methionine in marking infectious lesions.

Original language	English
Journal	American Journal of Nuclear Medicine and Molecular Imaging
Volume	6
Issue number	6
Pages (from-to)	286-300
Number of pages	15
ISSN	2160-8407
Publication status	Published - 2016

Access to Document

Utility of 11C-methionine and 11C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous 111In-labelled leukocytes, 99mTc-DPD, and 18F-FDGFinal published version, 2.37 MBLicence: Unspecified

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218858/pdf/ajnmmi0006-0286.pdfLicence: Unspecified

Fingerprint

Dive into the research topics of 'Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG'. Together they form a unique fingerprint.

Cite this

Afzelius, P., Alstrup, A. K., Schønheyder, H. C., Borghammer, P., Jensen, S. B., Bender, D., & Nielsen, O. L. (2016). Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG. American Journal of Nuclear Medicine and Molecular Imaging, 6(6), 286-300. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218858/pdf/ajnmmi0006-0286.pdf

Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model : comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG. / Afzelius, Pia; Alstrup, Aage Ko; Schønheyder, Henrik C et al.

In: American Journal of Nuclear Medicine and Molecular Imaging, Vol. 6, No. 6, 2016, p. 286-300.

Research output: Contribution to journal › Journal article › Research › peer-review

Afzelius, P, Alstrup, AK, Schønheyder, HC, Borghammer, P, Jensen, SB, Bender, D & Nielsen, OL 2016, 'Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG', American Journal of Nuclear Medicine and Molecular Imaging, vol. 6, no. 6, pp. 286-300. <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218858/pdf/ajnmmi0006-0286.pdf>

Afzelius P, Alstrup AK, Schønheyder HC, Borghammer P, Jensen SB, Bender D et al. Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG. American Journal of Nuclear Medicine and Molecular Imaging. 2016;6(6):286-300.

Afzelius, Pia ; Alstrup, Aage Ko ; Schønheyder, Henrik C et al. / Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model : comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG. In: American Journal of Nuclear Medicine and Molecular Imaging. 2016 ; Vol. 6, No. 6. pp. 286-300.

@article{2b8d9986fb4a420ba680ab36fa55d023,

title = "Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model: comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG",

abstract = "The aim of this study was to compare (11)C-methionine and (11)C-donepezil positron emission tomography (PET) with (111)In-labeled leukocyte and (99m) Tc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and (18)F-fluorodeoxyglucose ((18)F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus. The sequential scan protocol included Computed Tomography, (11)C-methionine and (11)C-donepezil PET, (99m) Tc-DPD and (111)In-labelled leukocytes scintigraphy, and (18)F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions, (18)F-FDG accumulated in 100%, (111)In-leukocytes in 79%, (11)C-methionine in 79%, (11)C-donepezil in 58%, and (99m) Tc-DPD in none. Overall, (18)F-FDG PET was superior to (111)In-leukocyte SPECT and (11)C-methionine in marking infectious lesions.",

author = "Pia Afzelius and Alstrup, {Aage Ko} and Sch{\o}nheyder, {Henrik C} and Per Borghammer and Jensen, {Svend B} and Dirk Bender and Nielsen, {Ole L}",

year = "2016",

language = "English",

volume = "6",

pages = "286--300",

journal = "American Journal of Nuclear Medicine and Molecular Imaging",

issn = "2160-8407",

publisher = "e-Century Publishing Corporation",

number = "6",

}

TY - JOUR

T1 - Utility of (11)C-methionine and (11)C-donepezil for imaging of Staphylococcus aureus induced osteomyelitis in a juvenile porcine model

T2 - comparison to autologous (111)In-labelled leukocytes, (99m) Tc-DPD, and (18)F-FDG

AU - Afzelius, Pia

AU - Alstrup, Aage Ko

AU - Schønheyder, Henrik C

AU - Borghammer, Per

AU - Jensen, Svend B

AU - Bender, Dirk

AU - Nielsen, Ole L

PY - 2016

Y1 - 2016

N2 - The aim of this study was to compare (11)C-methionine and (11)C-donepezil positron emission tomography (PET) with (111)In-labeled leukocyte and (99m) Tc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and (18)F-fluorodeoxyglucose ((18)F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus. The sequential scan protocol included Computed Tomography, (11)C-methionine and (11)C-donepezil PET, (99m) Tc-DPD and (111)In-labelled leukocytes scintigraphy, and (18)F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions, (18)F-FDG accumulated in 100%, (111)In-leukocytes in 79%, (11)C-methionine in 79%, (11)C-donepezil in 58%, and (99m) Tc-DPD in none. Overall, (18)F-FDG PET was superior to (111)In-leukocyte SPECT and (11)C-methionine in marking infectious lesions.

AB - The aim of this study was to compare (11)C-methionine and (11)C-donepezil positron emission tomography (PET) with (111)In-labeled leukocyte and (99m) Tc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and (18)F-fluorodeoxyglucose ((18)F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain of Staphylococcus aureus. The sequential scan protocol included Computed Tomography, (11)C-methionine and (11)C-donepezil PET, (99m) Tc-DPD and (111)In-labelled leukocytes scintigraphy, and (18)F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions, (18)F-FDG accumulated in 100%, (111)In-leukocytes in 79%, (11)C-methionine in 79%, (11)C-donepezil in 58%, and (99m) Tc-DPD in none. Overall, (18)F-FDG PET was superior to (111)In-leukocyte SPECT and (11)C-methionine in marking infectious lesions.

M3 - Journal article

C2 - 28078182

SN - 2160-8407

VL - 6

SP - 286

EP - 300

JO - American Journal of Nuclear Medicine and Molecular Imaging

JF - American Journal of Nuclear Medicine and Molecular Imaging

IS - 6

ER -