USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Hussam Hassan Nour El Din Auis; Fernando Geu Flores; Barbara Ann Halkier

doi:10.1007/978-1-60761-723-5_13

USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Hussam Hassan Nour El Din Auis, Fernando Geu Flores, Barbara Ann Halkier

98 Citations (Scopus)

Abstract

The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.

Original language	English
Title of host publication	Plant secondary metabolism engineering : methods and applications
Editors	Arthur Germano Fett-Neto
Number of pages	16
Volume	643
Publisher	Springer Science+Business Media
Publication date	2010
Pages	185-200
Chapter	13
DOIs	https://doi.org/10.1007/978-1-60761-723-5_13
Publication status	Published - 2010

Series	Methods in Molecular Biology
Volume	643

Access to Document

10.1007/978-1-60761-723-5_13

Cite this

Auis, H. H. N. E. D., Geu Flores, F., & Halkier, B. A. (2010). USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories. In A. G. Fett-Neto (Ed.), Plant secondary metabolism engineering: methods and applications (Vol. 643, pp. 185-200). Springer Science+Business Media. Methods in Molecular Biology Vol. 643 https://doi.org/10.1007/978-1-60761-723-5_13

USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories. / Auis, Hussam Hassan Nour El Din ; Geu Flores, Fernando ; Halkier, Barbara Ann.

Plant secondary metabolism engineering: methods and applications. ed. / Arthur Germano Fett-Neto. Vol. 643 Springer Science+Business Media, 2010. p. 185-200 (Methods in Molecular Biology, Vol. 643).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Auis, Hussam Hassan Nour El Din ; Geu Flores, Fernando ; Halkier, Barbara Ann. / USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories. Plant secondary metabolism engineering: methods and applications. editor / Arthur Germano Fett-Neto. Vol. 643 Springer Science+Business Media, 2010. pp. 185-200 (Methods in Molecular Biology, Vol. 643).

@inbook{3b348f017e964d17947d4f95febfe1b9,

title = "USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories",

abstract = "The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.",

author = "Auis, {Hussam Hassan Nour El Din} and {Geu Flores}, Fernando and Halkier, {Barbara Ann}",

year = "2010",

doi = "10.1007/978-1-60761-723-5_13",

language = "English",

volume = "643",

series = "Methods in Molecular Biology",

publisher = "Springer Science+Business Media",

pages = "185--200",

editor = "Fett-Neto, {Arthur Germano}",

booktitle = "Plant secondary metabolism engineering",

address = "Singapore",

}

TY - CHAP

T1 - USER cloning and USER fusion

T2 - the ideal cloning techniques for small and big laboratories

AU - Auis, Hussam Hassan Nour El Din

AU - Geu Flores, Fernando

AU - Halkier, Barbara Ann

PY - 2010

Y1 - 2010

N2 - The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.

AB - The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.

U2 - 10.1007/978-1-60761-723-5_13

DO - 10.1007/978-1-60761-723-5_13

M3 - Book chapter

C2 - 20552452

VL - 643

T3 - Methods in Molecular Biology

SP - 185

EP - 200

BT - Plant secondary metabolism engineering

A2 - Fett-Neto, Arthur Germano

PB - Springer Science+Business Media

ER -

USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Abstract

Access to Document

Fingerprint

Cite this