USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Hussam Hassan Nour El Din Auis; Fernando Geu Flores; Barbara Ann Halkier

doi:10.1007/978-1-60761-723-5_13

USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Hussam Hassan Nour El Din Auis, Fernando Geu Flores, Barbara Ann Halkier

98 Citations (Scopus)

Abstract

The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.

Original language	English
Title of host publication	Plant secondary metabolism engineering : methods and applications
Editors	Arthur Germano Fett-Neto
Number of pages	16
Volume	643
Publisher	Springer Science+Business Media
Publication date	2010
Pages	185-200
Chapter	13
DOIs	https://doi.org/10.1007/978-1-60761-723-5_13
Publication status	Published - 2010

Series	Methods in Molecular Biology
Volume	643

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10.1007/978-1-60761-723-5_13

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USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories. / Auis, Hussam Hassan Nour El Din ; Geu Flores, Fernando ; Halkier, Barbara Ann.
Plant secondary metabolism engineering: methods and applications. ed. / Arthur Germano Fett-Neto. Vol. 643 Springer Science+Business Media, 2010. p. 185-200 (Methods in Molecular Biology, Vol. 643).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Auis, Hussam Hassan Nour El Din ; Geu Flores, Fernando ; Halkier, Barbara Ann. / USER cloning and USER fusion : the ideal cloning techniques for small and big laboratories. Plant secondary metabolism engineering: methods and applications. editor / Arthur Germano Fett-Neto. Vol. 643 Springer Science+Business Media, 2010. pp. 185-200 (Methods in Molecular Biology, Vol. 643).

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USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

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