TY - CHAP
T1 - USER cloning and USER fusion
T2 - the ideal cloning techniques for small and big laboratories
AU - Auis, Hussam Hassan Nour El Din
AU - Geu Flores, Fernando
AU - Halkier, Barbara Ann
PY - 2010
Y1 - 2010
N2 - The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.
AB - The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.
U2 - 10.1007/978-1-60761-723-5_13
DO - 10.1007/978-1-60761-723-5_13
M3 - Book chapter
C2 - 20552452
VL - 643
T3 - Methods in Molecular Biology
SP - 185
EP - 200
BT - Plant secondary metabolism engineering
A2 - Fett-Neto, Arthur Germano
PB - Springer Science+Business Media
ER -