Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

Monica Hârţa; Doru Pamfil; Marin Ardelean; Carsten Pedersen; Rodica Pop; Mirela Cordea

Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

Monica Hârţa^*, Doru Pamfil, Marin Ardelean, Carsten Pedersen, Rodica Pop, Mirela Cordea

^*Corresponding author for this work

Abstract

The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

Original language	English
Journal	Romanian Biotechnological Letters
Volume	15
Issue number	SUPPL.2
Pages (from-to)	56-61
Number of pages	6
ISSN	1224-5984
Publication status	Published - 1 Mar 2010

Keywords

Aflp
DNA polymorphism
Fingerprinting techniques
Ginkgo biloba
Rapd

Cite this

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title = "Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species",

abstract = "The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.",

keywords = "Aflp, DNA polymorphism, Fingerprinting techniques, Ginkgo biloba, Rapd",

author = "Monica H{\^a}r{\c t}a and Doru Pamfil and Marin Ardelean and Carsten Pedersen and Rodica Pop and Mirela Cordea",

year = "2010",

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language = "English",

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T1 - Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

AU - Hârţa, Monica

AU - Pamfil, Doru

AU - Ardelean, Marin

AU - Pedersen, Carsten

AU - Pop, Rodica

AU - Cordea, Mirela

PY - 2010/3/1

Y1 - 2010/3/1

N2 - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

AB - The main goal of our study was to use two DNA fingerprinting techniques (RAPD and AFLP) for revealing the possible intraspecific genetic variation between Ginkgo biloba individuals grown in different sites. Ten of the twenty RAPD (Random Amplification of Polymorphic DNA ) decamer primers yielded scorable amplification patterns and generated polymorphic bands among the genotypes studied. Of the 165 AFLP (Amplified Fragment Length Polymorphism) primer combinations used, Ecs-AGG+MCG have revealed the largest number of polymorphic bands (96) among samples of Ginkgo biloba originating from Romania and Denmark. The least number of polymorfic bands (74) was detected using primer combination Ecs-AGG+MGA. Individuals of Ginkgo, analyzed by the techniques mentioned above, clustered into two main groups and the values of genetic similarity coefficients between data shows some differences at DNA molecular level.

KW - Aflp

KW - DNA polymorphism

KW - Fingerprinting techniques

KW - Ginkgo biloba

KW - Rapd

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M3 - Journal article

AN - SCOPUS:78651263380

SN - 1224-5984

VL - 15

SP - 56

EP - 61

JO - Romanian Biotechnological Letters

JF - Romanian Biotechnological Letters

IS - SUPPL.2

ER -

Use of genomic fingerprinting techniques for revealing DNA polymorphism in Ginkgo biloba L., A medicinal woody species

Abstract

Keywords

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